Evaluation location: Maryland, United States
Total foliar glycoalkaloid levels were determined by taking random samples consisting of 20-30 midlevel leaves from 4-6 sibs and boiling them in ethanol acidified with glacial acetic acid to extract the glycoalkaloids. The extracts were filtered out and analyzed by the TLC method to determine which glycoalkaloids were present and the GLC method to determine the amount and type of aglycone(s).