Evaluation location: Iowa, United States
Ploidy was determined by chromosome counts on root tips of germinating seeds and by flow cytometry. All subsp. falcata accessions were analyzed by both methods. All other accessions were analyzed with flow cytometry only. Chromosome counts were performed on root tips of newly germinated seeds as determined by Karp(1991), using ice-cold water for pretreatment. Counts were made microscopically on 5 to 10 cells per plants at 400 and 1000 times magnification. Data is published in: 'Ploidy Determination of Alflafa Germplasm Accessions Using Flow Cytometry'. E. Charles Brummer, Patricia M. Cazcarro, and Diane Luth. Crop Sci. 39:1202-1207(1999).