Evaluation location: Idaho, United States
Sugarbeet cyst nematode (Heterodera schachtii) screening done by Saad Hafez at Parma, Idaho, 1997. Accessions were planted in 500-cm3 pots (12 seeds per pot) containing naturally infested soil (2.6 eggs and larvae per cm3). Pots were arranged in a randomized complete block design with 6 replications in the greenhouse. Seedlings were carefully watered daily and fertilized twice with soluble 20-20-20. To control damping-off fungi, seedlings received weekly applications of Captan (50%) until harvest. Plants were harvested 7 + weeks after planting and soil was washed to recover females and cysts. Soil and root systems were evaluated separately for the number of white females and cysts, and the total number of females and cysts in root and soil was used in analysis. A score of 0 to 9 was assigned, where 0 = 100% resistant and 9 = susceptible, using the following calculation: ((Total Number of Cysts) / (Number of Cysts for the Susceptible Check))*9. Susceptible check WS-PM-9 had 113 cysts. Greenhouse planting date was Oct 13, 1997.