Evaluation location: Arkansas, United States
20 seeds were soaked in 5% NaOH for 16 hours, and then squashed with a coverslip. Each slide was examined under a microscope through 220X magnification for Neotphodium-like endophyte. Seed analysis for endophyte only determines it presences, not its viability. Seeds were collected in 1993 from Morocco, Spain and France. Percentage of seeds with endophyte is in the comment.