Evaluation location: Nebraska, United States
The flow cytometric techniques used was similar to that reported by Vogel and Arumuganathan. Differences include the use of Poa annua UM-184 as the internal standard. This Poa annua clone has a DNA content of 4.64 picograms DNS/2C nucleus. In most cases, ten seedlings from seeded populations were analyzed for DNA content. Clonal varieties were analyzed at least four times.