Evaluation location: Nebraska, United States
Flow Cytometry procedures described by Arumuganathan and Earle(1991) were used to determine DNA content per nuclei on Bromus accessions. Ten plants per accessions were analyzed with a 1000 nuclei scanned per plant. The mean DNA content per plant was based on the 1000 scanned nuclei. The DNA content was correlated to a few known chromosome numbers (Can. J. Plant Sci. 67:267-269(Jan 1987)) to determine ploidy of unknown accessions.