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Marker details for
CLS_S3_CONTIG9463-1-OP5
| Crop Name |
LETTUCE |
| Site |
Western Regional PI Station |
| Repeat Motif |
T/C |
| Polymorphic Type |
SEQ/SNP |
Evaluation Method
Genomic DNA (250 ng per sample) was used as template for SNP genotyping that was carried out in the UC Davis Genome Center. The Illumina's custom VeraCode GoldenGate Genotyping Kits (Illumina Inc, San Diego, CA, USA), were used to simultaneously genotype 384 loci in a single well of a standard 96-well microplate. The scanned data from BeadXpress Reader were analyzed with the Illumina's GenomeStudio (Version 1.0) software to generate genotype data for individual accessions using the automatic allele call feature. Manual re-clustering of homozygous and heterozygous clusters for some SNPs was performed when obvious error were observed in the automatic clustering. SNP markers are biallelic dominant markers. Each marker was scored for each sample: A, T, G, and C.
For a .xlsx file of the SNP data