ASTRAGALUS

Study Name: Nitrotoxin content of Astragalus accessions Comment: Nitrotoxin content as 3-NPA, spaced plant nursery, 10 reps 10 plant plots per entry over 2 years. For the methods see: Rumbaugh et al. 1985. Near Infrared reflectance spectroscopy screening of Astragalus spp. for nitro-toxins. Proceedings XV International Grasslands Congress, Kyoto, Japan. p315-316

Study Name: Nitrotoxin content of Astragalus accessions Comment: Nitrotoxin content as NO2, spaced plant nursery, 10 reps of 10 plant plots per entry over 2 years. For the methods see: Rumbaugh et al. 1985. Near Infrared reflectance spectoscopy screening of Astragalus spp. for nitro-toxins. Proceedings XV International Grasslands Congress, Kyoto, Japan. p315-316

Pictures taken of nursery/greenhouse accessions

Study Name: Elicitation of 5 isoflavonoid phytoalexins by A. cicer. Comment: The means of 5 isoflavenoids (Mucronulatol, Unknown, Cajanin Maackiain and Astraciceran) accumulated in response to Helminthosporium carbonum. The data is recorded as the t total of the 5 means in micrograms / innoculation droplet after incubation 48 hours at 28 degrees C in light.

100 seed weight at the Western Regional Plant Introduction Station, Pullman Washington, USA

A 3 year survey of the bee associates of flowering accessions of Astragalus and Onobrychis species was conducted in open-pollinated field nurseries at Central Ferry, Washington to gain insight into the potential for bee-mediated inter-accession out-crossing in these nurseries. Such nurseries are used by genebank managers at the USDA-ARS Western Regional Plant Introduction Station (WRPIS), Pullman, Washington, to multiply seed of accessions low in viability and/or supply. Fifty-two species of bees (Hymenoptera) in the Andrenidae (4 species), Apidae (18), Colletidae (1), Halictidae (5), and Megachilidae (24) were recorded. Species-rich genera were Bombus (8 species) and Osmia (16). One undescribed species of Osmia was collected. The bee fauna was more diverse on Astragalus (45 bee species) compared to Onobrychis (29 species). Among the 24 Astragalus and 13 Onobrychis species censused, bee diversity was highest on A. bungeanus Boiss. (24 bee species), A. chaborasicus Boiss. & Hausskn. (24 species), A. miser var. serotinus (A. Gray ex J.G. Cooper) Barney (14 species), A. cicer L. (13 species), A. schistosus Boiss. & Hohen. (12 species), A. onobrychis L. (11 species), A. podolobus Boiss. & Hohen. (11 species), and Onobrychis viciifolia Scop. (10 species). The presence of a rich bee fauna in WRPIS nurseries at Central Ferry, including documented pollinators of the allogamous species A. cicer and O. viciifolia, could complicate efforts to prevent hybridization and maintain genetic integrity of genebank accessions of allogamous plant species. Our results support a 1993 decision by WRPIS curators to discontinue the practice of clustering accessions of single species of Astragalus and Onobrychis in open-pollinated nurseries to regenerate seed.

Central Ferry farm picture.

An Excel spread sheet of Clement's data from Central Ferry farm in Washington state(.xls).

Collection pictures from the Richard C. Johnson, Harold Bockelman, Korzhenevsky, V., Boguslavsky, R. collection in 1999 from Krym, Ukraine.

Collection pictures taken in 2000 from the Kazakstan trip made by Rich Hannan, Stephanie Greene, A. Khusainov, A. Afonin, and N. Dzyubenko.

Collection pictures from Fred Meuhlbauer and Walt Kaiser 2004 trip to Georgia.

For a Powerpoint view of 24 pictures of the Georgia collection click here. It is 7793 KB in size.

Pictures taken from plants growing in the Pullman nursery in 2000

Images of plants grown for seed increase at the Observatory Hill field at WSU, Pullman, WA. Nursery was established 5/25 to 5/31/2011 using seedlings started in the greenhouse.

Images of pods and/or seed taken from the original seed packet. Seed used for planting.

Images of plants grown for seed increase at the Tukey Orchard plot at WSU, Pullman, WA. Nursery was established 5/19/2010 except for the Purshia which was established in 2009. Seedlings started in the greenhouse were planted for all accessions.