MAIZE

Reported in Crop Science Registration text. See citation in narrative.

Bulk of regenerations done in Mexico.

Lots created from bulks of 2 or more lots grown in Ames, Iowa only.

Information provided in the "Catalogo de Recursos Geneticos de Maiz de Chile', INIA, 1990.

Extracted from CIMMYT GRIN-Global website.

Data from ARGENTIN.DBF on LAMP-CIMMYT CD JUNE, 1995.

Data from MEX_ALL.DBF on LAMP-CIMMYT CD JUNE, 1995.

Study Name: CIMMYT MAIZE BANK PASSPORT RACE DATA

Extracted from the information located on the World Wide Web at:http://singer.cgiar.org/ .IPGRI holds the copyright.

Observations made by germplasm users.

Second observation of the same type from a second germplasm user.

Third observation of the same type from a third germplasm users.

Typical Races found in Catalogo de Recursos Geneticos de Maiz de Chile.

A breeder-targeted distillation of the genetic diversity of more than 12,000 Latin American maize landrace accessions, based on stage 1 and 2 evaluation trials of the Latin American Maize Project (LAMP), 1986-88. The CD and accompanying publication cantain relevant data, designated core accessions (20% of the total collection), and graphical representations of the groupings formed using cluster analysis.

Originally 303 diverse inbreds accumulated by Dr. Major Goodman.

Information documented based on curator consultion with a group of maize experts. The committee of experts changes over time and in relation to the observation. This observation may be supplimented or replaced at any time when additional experimental evidence is available.

Observations made in the scientific literature during searches by the Maize curator.

General observations made by the Maize curator about an accession not connected with a specific growout. Observations were made by the curator from 1980 until the present, but may include documentation obtained before that time.

Study Name: Screening for Diplodia zeae stalk rot resistance. Experiment Type: Field FIELD

Comment: Information provided by donor under unknown conditions

Comment: Observations recorded from donated materials.

2000 Ames ECB1 evaluations.

2001 Ames ECB1 evaluations.

Study Name: First Generation European Corn Borer Resistance Screening Experiment Type: Field FIELD Exp. Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Elevation: 300 meters Year started: //1987 Year seeded: //1987 Year planted: //1987

Study Name: First Generation European Corn Borer Resistance Screening Experiment Type: Field FIELD Exp. Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Elevation: 300 meters Year started: //1988 Year seeded: //1988 Year planted: //1988

Study Name: First Generation European Corn Borer Resistance Screening Experiment Type: Field FIELD Exp. Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year started: //1989 Year tested: //1989

Study Name: First Generation European Corn Borer Resistance Screening Experiment Type: Field

Study Name: First Generation European Corn Borer Resistance Screening Experiment Type: Field

Study Name: First Generation European Corn Borer Resistance Screening Experiment Type: Field

Study Name: First Generation European Corn Borer Resistance Screening Experiment Type: Field

Study Name: First Generation European Corn Borer Resistance Screening Experiment Type: Field FIELD Exp. Design: RCB Year seeded: //1991 Year planted: //1991

Study Name: First Generation European Corn Borer Resistance Screening Experiment Type: Field

Study Name: First Generation European Corn Borer Resistance Screening Experiment Type: Field

Study Name: First Generation European Corn Borer Resistance Screening Experiment Type: Field

Not a strong ECB1 damage year. Susceptible check Wf9 = 7.75 over 4 replications. Resistant check CI31A = 2 over 5 replications.

The test was planted on two dates. Rain prevented planting the test on one date. Ranges 1-12 were planted on 6/5/95 and ranges 13-36 were planted on 6/7/95. Plots were thinned to 6 plants per row on 6/23/95. Plots were infested on two days with approximately 100 larvae per plant each day on 7/5/95 and 7/11/95. Ratings were performed by R. L. Wilson on 8/1/95. Susceptible check Wf9 rated 8.16 over 6 replications. Resistant check CI31A rated 1.33 over 6 replications.

This evaluation was jab-planted on June 12. Plants (6/accession) were infested with 4 shots (ca. 200 larvae) total from July 5 to 10. R.L. Wilson rated the leaf damage on July 31.

This plot was jab-planted on June 2, 1997. Rows were thinned to 6 plants per accession on June 18. The plots were infested July 8-10, 1997. Each plant received 5 shots total or up to 375 neonate ECB larvae. Infestation was below average. Insects were obtained from J.Dyer of the Corn Insects Lab. R.L. Wilson rated the leaf damage.

1998 Ames ECB1 evaluations.

1999 Ames ECB1 evaluations.

Study Name: First Generation European Corn Borer Resistance Screening Experiment Type: Field

A total of 1,167 lines were evaluated in tests consisting of from 1 rep in 1 year to several hundred reps spread over multiple years. Lines were planted in single-row plots consisting of five 3-plant hills (40 inches. between hills) or in single-row plots containing 16 plants (about 10 inches between plants). Rows were spaced by 40 inches.

Usually 6 to 16 plants at midwhorl stage of development were infested with 4 egg masses giving an infestation of approximately 80-100 eggs per plant. In most years 2 masses were placed on plants in each of 2 dates approximately 3-7 days apart when lines were 18-32 inches in extended leaf height. At 3-4 weeks after egg hatch lines were rated for damage on a plot basis.

A 9 class rating scale was used with 1-2 considered highly resistant, 3-4 resistant, 5-6 intermediate in resistance, and 7-9 were highly susceptible.

The evaluations were done in Ames, Iowa. Ten plants per accession were infested with about 300 European corn borer neonates per plant (distributed over 6 nodes) after pollen shed began. After 60 days, plants were split lengthwise and total inches of larval tunneling per stalk was recorded. The average inches of tunneling was calculated for each accession and then was compared to the year's overall average inches of tunneling in B52 a resistant check. For purposes of reporting data to GRIN, we converted centimeters of tunneling as a percent of the B52 resistant check tunneling. Ratings of resistant (1-3) were assigned to accessions which had centimeters of tunneling less than or equal to B52 (<100%), ratings of intermediate (4-6) were assigned to accessions which equalled 100-199% of the check, and ratings of susceptible (7-9) were assigned to accessions which were greater than 200% of the check. In 1993, 200 accessions were planted in test W9307. 53 were not tested due to field flooding. B52 averaged 30 cm of tunnelling in 1993. An unusually large number of accessions tested resistant in 1993 and is probably due to poor weather for the European corn borer.

The evaluations were done in Ames, Iowa. Ten plants per accession were infested with about 300 European corn borer neonates per plant (distributed over 6 nodes) after pollen shed began. After 60 days, plants were split lengthwise and total inches of larval tunneling per stalk was recorded. The average inches of tunneling was calculated for each accession and then was compared to the year's overall average inches of tunneling in B52 a resistant check. For purposes of reporting data to GRIN, we converted centimeters of tunneling as a percent of the B52 resistant check tunneling. Ratings of resistant (1-3) were assigned to accessions which had centimeters of tunneling less than or equal to B52 (<100%), ratings of intermediate (4-6) were assigned to accessions which equalled 100-199% of the check, and ratings of susceptible (7-9) were assigned to accessions which were greater than 200% of the check. In 1994, 200 accessions were planted in test W9407. 6 were not tested due to storms. B52 averaged 13.5 cm of tunnelling in 1994.

The evaluations were done in Ames, Iowa. The test plot was planted on 5/19/95. The field was thinned on 6/6/95. Ten plants per accession were infested with about 300 European corn borer neonates per plant (distributed over 6 nodes) when pollen shed began. Infestations were made from 7/5/95 through 8/9/95. 60 days after infestation, stalks were split in half lengthwise with a band saw and the total inches of larval tunneling per stalk was recorded. Stalks were split from 8/8/95 through 10/18/95. The ratings were obtained as follows: The average inches of tunneling was calculated for each accession and then was compared to this year's overall average inches of tunneling in B52 (2.33 inches), our resistant check. Inches of tunneling was then converted to a percent of the check. Resistant accessions were < 100 % of check, intermediate accessions were 100 to 199 % of the check, and susceptible accessions were > 200 % of the check.

The evaluations were done in Ames, Iowa.

The plot was planted via 2-row planter on May 12, 1997. Each row was thinned to 10 plants per row just prior to infestation. Each plant was infested with ca. 50 ECB neonates at pollen shed at 6 different nodes (300 neonates total per plant); infestations were done during the month of July. Plants were split lengthwise with a band saw and total cm of tunneling was counted; rating was done at least 60 days after infestation, from July 31 to October 16, 1997. Overall average inches of tunneling in the resistant check B52 was 10 cm (10 rows of 10 plants/row). There were 0 accessions that rated a "1" - % of check < 33. There was 1 accession that rated a "2" - % of check > 33. There was 1 accession that rated a "3" - % of check > 67. There were 3 accessions that rated a "4" - % of check > 100. There were 4 accessions that rated a "5" - % of check > 133. There were 5 accessions that rated a "6" - % of check > 167. There were 12 accessions that rated a "7" - % of check > 200. There were 8 accessions that rated an "8" - % of check > 233. There were 166 accessions that rated a "9" - % of check > 267.

1999 Ames ECB2 evaluation.

Study Name: Second Generation European Corn Borer Resistance Screening Experiment Type: Field FIELD

Study Name: EMBRAPA MAIZE BANK PASSPORT RACE DATA in EMBRAPA.dbf file on 1995 LAMP CD.

Nursery was grown at the Pioneer facilities at Woodland, Iowa to evaluate for maize head smut resistance.

350 maize accessions from the NCRPIS collection were evaluated in 2000 for resistance to northern leaf blight (NLB), caused by Exserohilum turcicum race O and eyespot, caused by Kabatiella zeae. Plots were planted on 9-May-2000 at the Hinds Research farm, Ames, IA. The trial included two replicates of single rows with approximately 20-25 plants per row. Rows were spaced 0.76 m apart. Plants were inoculated on 27-JUN and 03-JUL. The first half of each row was inoculated with eyespot and the second half with northern leaf blight. Initial eyespot ratings were made on 25-JUL with a second rating made between 21-AUG and 24-AUG. During the initial eyespot rating, an average lesion diameter (in mm) was measured. The score for the latter rating date was used to determine the final accession rating except for early maturing accessions where the first score was used. Each plot was given an overall score based on the average disease development on all plants in the plot. Scores for the two replications were averaged to obtain an overall rating for each accession. First Eyespot Rating (0-5 scale):0 = no disease;1 = few lesions per leaf;2 = areas of 20 or more lesions on leaf;3 = over 50 lesions per leaf;4 = 200 or more lesions per leaf;5 = some areas of leaf dead due to eyespot. Second Eyespot Rating (0-9 scale); expansion of 0-5 scale:General categories:1-3 = low disease;4-6 = moderate disease;7-9 = severe disease. Specific categories:0 = no disease;1 = few lesions per leaf;2 = moderate number of lesions per leaf below ear;3 = numerous lesions on leaves below the ear, but no evidence of lesion coalescence;4 = numerous lesions with some patches of leaf tissue killed below the ear;5 = whole leaves killed up to the ear and some disease evident above the ear;6 = heavy disease over whole plant, some leaves above the ear severely infected;7 = heavy disease over whole plant and some leaves above the ear killed;8 = all leaf tissue above ear nearly completely diseased;9 = plants dead.

The Goodman maize diversity panel was grown in Urbana, IL in single-row plots during the 2016 and 2017 growing season. Disease ratings of two replicates was completed in 2016, and ratings of 2 replicates of 223 lines was completed in 2017. Ratings were performed 3-4 times at approximate 2-week intervals. AUDPC was calcualted for each plot and log(10) transformed to normalize the data. The original value recorded in GRIN is the BLUP for each accession. A relative susceptibility rating (1 = resistant, 9 = susceptible) was assigned to each accession by the maize curator (VMB) based on the BLUPs. doi: 10.3835/plantgenome2018.06.0045

Study Name: Maize Increase Descriptions from older Converted NC-7 data. Latitude: 42 Degrees 3 Minutes N Longitude: 93 Degrees 53 Minutes W Date Planted:05/18/1955 Locations: Field G, Field J, Field F. Comment: Data collected on increase plots on inventory grown or harvested in Ames in 1955.

Specific field plant health notes for seedborne maize pathogens by accession in the nursery have been recovered from the note archive as of 8-Feb-2018.

Study Name: Maize Increase Descriptions from older Converted NC-7 data. Latitude: 42 Degrees 3 Minutes N Longitude: 93 Degrees 53 Minutes W Date Planted:05/14/1956 Locations: 10-Acre Field. Comment: Data collected on increase plots on inventory grown or harvested in Ames in 1956.

Specific field plant health notes for seedborne maize pathogens by accession in the nursery have been recovered from the note archive as of 8-Feb-2018.

Study Name: Maize Increase Descriptions from older Converted NC-7 data. Latitude: 42 Degrees 3 Minutes N Longitude: 93 Degrees 53 Minutes W Date Planted:05/21/1957,replanted 06/06/1957. Locations: Field I. Field J. Comment: Data collected on increase plots on inventory grown or harvested in Ames in 1957.

Specific field plant health notes for seedborne maize pathogens by accession in the nursery have been recovered from the note archive as of 8-Feb-2018.

Study Name: Maize Increase Descriptions from older Converted NC-7 data. Latitude: 42 Degrees 3 Minutes N Longitude: 93 Degrees 53 Minutes W Year planted: //1958 Comment: Data collected on increase plots on inventory grown or harvested in Ames in 1958.

Specific field plant health notes for seedborne maize pathogens by accession in the nursery have been recovered from the note archive as of 8-Feb-2018.

Study Name: Maize Increase Descriptions from older Converted NC-7 data. Latitude: 42 Degrees 3 Minutes N Longitude: 93 Degrees 53 Minutes W Year planted: //1959 Comment: Data collected on increase plots on inventory grown or harvested in Ames in 1959.

Specific field plant health notes for seedborne maize pathogens by accession in the nursery have been recovered from the note archive as of 8-Feb-2018.

Study Name: Maize Increase Descriptions from older Converted NC-7 data. Latitude: 42 Degrees 3 Minutes N Longitude: 93 Degrees 53 Minutes W Year planted: //1960 Comment: Data collected on increase plots on inventory grown or harvested in Ames in 1960.

Specific field plant health notes for seedborne maize pathogens by accession in the nursery have been recovered from the note archive as of 8-Feb-2018.

Study Name: Maize Increase Descriptions from older Converted NC-7 data. Latitude: 42 Degrees 3 Minutes N Longitude: 93 Degrees 53 Minutes W Year planted: //1961 Comment: Data collected on increase plots on inventory grown or harvested in Ames in 1961.

Specific field plant health notes for seedborne maize pathogens by accession in the nursery have been recovered from the note archive as of 8-Feb-2018.

Study Name: Maize Increase Descriptions from older Converted NC-7 data. Latitude: 42 Degrees 3 Minutes N Longitude: 93 Degrees 53 Minutes W Year planted: //1962 Comment: Data collected on increase plots on inventory grown or harvested in Ames in 1962.

Specific field plant health notes for seedborne maize pathogens by accession in the nursery have been recovered from the note archive as of 8-Feb-2018.

Study Name: Maize Increase Descriptions from older Converted NC-7 data. Latitude: 42 Degrees 3 Minutes N Longitude: 93 Degrees 53 Minutes W Year planted: //1963 Comment: Data collected on increase plots on inventory grown or harvested in Ames in 1963.

Specific field plant health notes for seedborne maize pathogens by accession in the nursery have been recovered from the note archive as of 8-Feb-2018.

Study Name: Maize Increase Descriptions from older Converted NC-7 data. Latitude: 42 Degrees 3 Minutes N Longitude: 93 Degrees 53 Minutes W Year planted: //1964 Comment: Data collected on increase plots on inventory grown or harvested in Ames in 1964.

Specific field plant health notes for seedborne maize pathogens by accession in the nursery have been recovered from the note archive as of 8-Feb-2018.

Study Name: Maize Increase Descriptions from older Converted NC-7 data. Latitude: 42 Degrees 3 Minutes N Longitude: 93 Degrees 53 Minutes W Year planted: //1965 Comment: Data collected on increase plots on inventory grown or harvested in Ames in 1965.

Specific field plant health notes for seedborne maize pathogens by accession in the nursery have been recovered from the note archive as of 8-Feb-2018.

Study Name: Maize Increase Descriptions from older Converted NC-7 data. Latitude: 42 Degrees 3 Minutes N Longitude: 93 Degrees 53 Minutes W Year planted: //1966 Comment: Data collected on increase plots on inventory grown or harvested in Ames in 1966.

Specific field plant health notes for seedborne maize pathogens by accession in the nursery have been recovered from the note archive as of 8-Feb-2018.

Study Name: Maize Increase Descriptions from older Converted NC-7 data. Latitude: 42 Degrees 3 Minutes N Longitude: 93 Degrees 53 Minutes W Year planted: //1967 Comment: Data collected on increase plots on inventory grown or harvested in Ames in 1967.

Specific field plant health notes for seedborne maize pathogens by accession in the nursery have been recovered from the note archive as of 8-Feb-2018.

Study Name: Maize Increase Descriptions from older Converted NC-7 data. Latitude: 42 Degrees 3 Minutes N Longitude: 93 Degrees 53 Minutes W Year planted: //1968 Comment: Data collected on increase plots on inventory grown or harvested in Ames in 1968.

Specific field plant health notes for seedborne maize pathogens by accession in the nursery have been recovered from the note archive as of 8-Feb-2018.

Study Name: Maize Increase Descriptions from older Converted NC-7 data. Latitude: 42 Degrees 3 Minutes N Longitude: 93 Degrees 53 Minutes W Year planted: //1969 Comment: Data collected on increase plots on inventory grown or harvested in Ames in 1969.

Specific field plant health notes for seedborne maize pathogens by accession in the nursery have been recovered from the note archive as of 8-Feb-2018.

Study Name: Maize Increase Descriptions from older Converted NC-7 data. Latitude: 42 Degrees 3 Minutes N Longitude: 93 Degrees 53 Minutes W Year planted: //1970 Comment: Data collected on increase plots on inventory grown or harvested in Ames in 1970.

Specific field plant health notes for seedborne maize pathogens by accession in the nursery have been recovered from the note archive as of 8-Feb-2018.

Study Name: Maize Increase Descriptions from older Converted NC-7 data. Latitude: 42 Degrees 3 Minutes N Longitude: 93 Degrees 53 Minutes W Year planted: //1971 Comment: Data collected on increase plots on inventory grown or harvested in Ames in 1971.

Specific field plant health notes for seedborne maize pathogens by accession in the nursery have been recovered from the note archive as of 8-Feb-2018.

Study Name: Maize Increase Descriptions from older Converted NC-7 data. Latitude: 42 Degrees 3 Minutes N Longitude: 93 Degrees 53 Minutes W Year planted: //1972 Comment: Data collected on increase plots on inventory grown or harvested in Ames in 1972.

Specific field plant health notes for seedborne maize pathogens by accession in the nursery have been recovered from the note archive as of 8-Feb-2018.

Study Name: Maize Increase Descriptions from older Converted NC-7 data. Latitude: 42 Degrees 3 Minutes N Longitude: 93 Degrees 53 Minutes W Year planted: //1973 Comment: Data collected on increase plots on inventory grown or harvested in Ames in 1973.

Specific field plant health notes for seedborne maize pathogens by accession in the nursery have been recovered from the note archive as of 8-Feb-2018.

Study Name: Maize Increase Descriptions from older Converted NC-7 data. Latitude: 42 Degrees 3 Minutes N Longitude: 93 Degrees 53 Minutes W Year planted: //1974 Comment: Data collected on increase plots on inventory grown or harvested in Ames in 1974.

Specific field plant health notes for seedborne maize pathogens by accession in the nursery have been recovered from the note archive as of 8-Feb-2018.

Study Name: Maize Increase Descriptions from older Converted NC-7 data. Latitude: 42 Degrees 3 Minutes N Longitude: 93 Degrees 53 Minutes W Year planted: //1975 Comment: Data collected on increase plots on inventory grown or harvested in Ames in 1975.

Specific field plant health notes for seedborne maize pathogens by accession in the nursery have been recovered from the note archive as of 8-Feb-2018.

Study Name: Maize Increase Descriptions from older Converted NC-7 data. Latitude: 42 Degrees 3 Minutes N Longitude: 93 Degrees 53 Minutes W Year planted: //1976 Comment: Data collected on increase plots on inventory grown or harvested in Ames in 1976.

Specific field plant health notes for seedborne maize pathogens by accession in the nursery have been recovered from the note archive as of 8-Feb-2018.

Study Name: Maize Increase Descriptions from older Converted NC-7 data. Latitude: 42 Degrees 3 Minutes N Longitude: 93 Degrees 53 Minutes W Year planted: //1977 Comment: Data collected on increase plots on inventory grown or harvested in Ames in 1977.

Specific field plant health notes for seedborne maize pathogens by accession in the nursery have been recovered from the note archive as of 8-Feb-2018.

Study Name: Maize Increase Descriptions from older Converted NC-7 data. Latitude: 42 Degrees 3 Minutes N Longitude: 93 Degrees 53 Minutes W Year planted: //1978 Comment: Data collected on increase plots on inventory grown or harvested in Ames in 1978.

Specific field plant health notes for seedborne maize pathogens by accession in the nursery have been recovered from the note archive as of 8-Feb-2018.

Study Name: Maize Increase Descriptions from older Converted NC-7 data. Latitude: 42 Degrees 3 Minutes N Longitude: 93 Degrees 53 Minutes W Year planted: //1979 Comment: Data collected on increase plots on inventory grown or harvested in Ames in 1979.

Specific field plant health notes for seedborne maize pathogens by accession in the nursery have been recovered from the note archive as of 8-Feb-2018.

Study Name: Maize Increase Descriptions from older Converted NC-7 data. Latitude: 42 Degrees 3 Minutes N Longitude: 93 Degrees 53 Minutes W Year planted: 12-MAY-1980 Comment: Data collected on increase plots on inventory grown or harvested in Ames in 1980.

Specific field plant health notes for seedborne maize pathogens by accession in the nursery have been recovered from the note archive as of 8-Feb-2018.

Study Name: Maize Increase Descriptions from older Converted NC-7 data. Latitude: 42 Degrees 3 Minutes N Longitude: 93 Degrees 53 Minutes W Year planted: 20-MAY-1981 Comment: Data collected on increase plots on inventory grown or harvested in Ames in 1981.

Specific field plant health notes for seedborne maize pathogens by accession in the nursery have been recovered from the note archive as of 8-Feb-2018.

Study Name: Maize Increase Descriptions from older Converted NC-7 data. Latitude: 42 Degrees 3 Minutes N Longitude: 93 Degrees 53 Minutes W Year planted: 02-JUN-1982 Comment: Data collected on increase plots on inventory grown or harvested in Ames in 1982.

Specific field plant health notes for seedborne maize pathogens by accession in the nursery have been recovered from the note archive as of 8-Feb-2018.

Study Name: Maize Increase Descriptions from older Converted NC-7 data. Latitude: 42 Degrees 3 Minutes N Longitude: 93 Degrees 53 Minutes W Year planted: //1983 Comment: Data collected on increase plots on inventory grown or harvested in Ames in 1983.

Specific field plant health notes for seedborne maize pathogens by accession in the nursery have been recovered from the note archive as of 8-Feb-2018.

Plants were started in the greenhouse in early spring then transplanted to the field to see if this would aid in maturity before a killing frost.

Study Name: Maize Increase Descriptions from older Converted NC-7 data. Latitude: 42 Degrees 3 Minutes N Longitude: 93 Degrees 53 Minutes W Year planted: //1984 Comment: Data collected on increase plots on inventory grown or harvested in Ames in 1984.

Specific field plant health notes for seedborne maize pathogens by accession in the nursery have been recovered from the note archive as of 8-Feb-2018.

Study Name: Maize Increase Descriptions from older Converted NC-7 data. Latitude: 42 Degrees 3 Minutes N Longitude: 93 Degrees 53 Minutes W Year planted:First 27-APR-1985, Last 07-JUN-1985. Comment: Data collected on increase plots on inventory grown or harvested in Ames in 1985.

Specific field plant health notes for seedborne maize pathogens by accession in the nursery have been recovered from the note archive as of 8-Feb-2018.

Study Name: Maize Increase Descriptions from older Converted NC-7 data. Latitude: 42 Degrees 3 Minutes N Longitude: 93 Degrees 53 Minutes W Year planted:First 23-APR-1986, Last 9-JUN-1986. Comment: Data collected on increase plots on inventory grown or harvested in Ames in 1986.

Specific field plant health notes for seedborne maize pathogens by accession in the nursery have been recovered from the note archive as of 8-Feb-2018.

Study Name: Maize Increase Descriptions from older Converted NC-7 data. Latitude: 42 Degrees 3 Minutes N Longitude: 93 Degrees 53 Minutes W Year planted: //1987 Comment: Data collected on increase plots on inventory grown or harvested in Ames in 1987.

Specific field plant health notes for seedborne maize pathogens by accession in the nursery have been recovered from the note archive as of 8-Feb-2018.

Second direct seeded maize 1988 planting on 03-MAY-1988 (MAIZE.INC.AMES.1988.PLANTING2),. First pollination date was on 03-JUN-1988.DESCRIPTION of ALL 1988 maize plantings: Study Name: Maize Increase Descriptions from older Converted NC-7 data. Latitude: 42 Degrees 3 Minutes N Longitude: 93 Degrees 53 Minutes W Year planted:First 02-MAY-1988 (MAIZE.INC.AMES.1988.PLANTING2), Argentine direct seed 03-MAY-1988 (MAIZE.INC.AMES.1988.PLANTING3), Last May planting 06-MAY-1988 (MAIZE.INC.AMES.1988.PLANTING4). Argentine transplant 60000 rows were transplanted to field 11-MAY-1988 (MAIZE.INC.AMES.1988.PLANTING1). Last maize planting 03-JUN-1988 (MAIZE.INC.AMES.1988.PLANTING5). First pollination date was on 03-JUN-1988. Comment: Data collected on increase plots on inventory grown or harvested in Ames in 1988.

Specific field plant health notes for seedborne maize pathogens by accession in the nursery have been recovered from the note archive as of 8-Feb-2018.

Study Name: Maize Increase Descriptions from older Converted NC-7 data. Latitude: 42 Degrees 3 Minutes N Longitude: 93 Degrees 53 Minutes W Year planted:First 10-MAY-1989 Comment: Data collected on increase plots on inventory grown or harvested in Ames in 1989.

Specific field plant health notes for seedborne maize pathogens by accession in the nursery have been recovered from the note archive as of 8-Feb-2018.

Study Name: Maize Increase Descriptions from older Converted NC-7 data. Latitude: 42 Degrees 3 Minutes N Longitude: 93 Degrees 53 Minutes W Year planted: //1990 Comment: Data collected on increase plots on inventory grown or harvested in Ames in 1990.

This was the first year the station pathologist walked the nursery looking at each accession with the purpose of noting seed transmissible maize diseases and exotic diseases not known to occur in Iowa. Pathology notes were not extensive, but no exotic diseases were noted. Specific notes on Stewart's wilt and Goss's wilt were obtained. None of the exotic downy mildews were observed. The nursery was not examined for possible virus infected plants

Study Name: Maize Increase Descriptions from older Converted NC-7 data. Latitude: 42 Degrees 3 Minutes N Longitude: 93 Degrees 53 Minutes W Date planted: //28-MAY-1991 Comment: Data collected on increase plots on inventory grown or harvested in Ames in 1991.

Pathology notes were not extensive, but no exotic diseases were noted. Specific notes on Stewart's wilt and Goss's wilt were obtained. None of the exotic downy mildews were observed. The nursery was not examined for possible virus infected plants

Study Name: Maize Increase Descriptions from older Converted NC-7 data. Latitude: 42 Degrees 3 Minutes N Longitude: 93 Degrees 53 Minutes W Year planted: //1992 Comment: Data collected on increase plots on inventory grown or harvested in Ames in 1992.

Pathology notes were not extensive, but no exotic diseases were noted. Specific notes on Stewart's wilt and Goss's wilt were obtained. None of the exotic downy mildews were observed. The nursery was not examined for possible virus infected plants

Study Name: Maize Increase Descriptions from older Converted NC-7 data. Year planted: 1994. Data collected on increase plots on inventory grown or harvested in Ames in 1994.

Pathology notes were not extensive, but no exotic diseases were noted. Specific notes on Stewart's wilt and Goss's wilt were obtained. None of the exotic downy mildews were observed. The nursery was not examined for possible virus infected plants

Study Name: Observations from 1995 Maize increase. Year planted: 1995. Planted 18-May-1995 in NCRPIS historic field NF4.

Data collected on increase plots on inventory grown or harvested in Ames in 1995.

Pathology notes were not extensive, but no exotic diseases were noted. Specific notes on Stewart's wilt and Goss's wilt were obtained. None of the exotic downy mildews were observed. The nursery was not examined for possible virus infected plants

Study Name: Observations from 1996 Maize increase. Year planted: 1996. SF2 was planted 05/21/1996 and 5/22/1996. SF1 was planted 06/18/1996. The first pollination date was 07/07/1996 and was used for all planting dates.

Pathology notes were extensive. No exotic diseases were noted. Where each lot was observed, specific notes on Stewart's wilt and Goss's wilt and other diseases including virus diseases were obtained and recorded for each accession inventory. None of the exotic downy mildews were observed.

Environment for accessions grown in Ames during the summer of 1997. Planted in Plant Introduction fields D, NF4, and J5. Planting date 09-May-1997. The first pollination date was on 28-JUN-1997.

The pathologist did examine the fields for Stewart's wilt, but observations were not comprehensive for other diseases. Therefore, only observations were captured into the database for targeted organisms such as Stewart's wilt.

Environment for accessions grown in Ames during the summer of 1998. Planted in Plant Introduction fields. Planting date 11-MAY-1998. The first pollination date was on 01-JUL-1998.

Pathology notes were extensive. No exotic diseases were noted. Where each lot was observed, specific notes on Stewart's wilt and Goss's wilt and other diseases including virus diseases were obtained and recorded for each accession inventory. None of the exotic downy mildews were observed.

Environment for accessions grown in Ames during the summer of 1999. Planted in Plant Introduction fields A,F. Planting date May 26, 1999. The first pollination date was on 10-JUL-1999. On 31-JUL-1999, a windstorm from the west blew many accessions down. Field A plants were blown across rows while field F was blown down the rows. First frost and freeze on the morning of 04-OCT-1999.

Pathology notes were extensive. No exotic diseases were noted. Where each lot was observed, specific notes on Stewart's wilt and Goss's wilt and other diseases including virus diseases were obtained and recorded for each accession inventory. None of the exotic downy mildews were observed.

Environment for accessions grown in Ames during the summer of 2000. Planted in Plant Introduction fields D,E,and G. Planting date 05-MAY-2000. The first pollination date was on 30-JUN-2000. First frost and freeze on the morning of 00-___-2000.

Data was collected on increase plots on inventory grown or harvested in Ames in 2000.

Pathology notes were not extensive, but no exotic diseases were noted. Specific notes on Stewart's wilt and Goss's wilt were obtained. None of the exotic downy mildews were observed. The nursery was not examined for possible virus infected plants

Environment for accessions grown in Ames during the summer of 2001. Planted in Plant Introduction fields A and J-11. Planting date was 10-MAY-2001 for field A. Planting date was 18-MAY-2001 for J-11. The first pollination date was on 08-JUL-2001. First frost on the morning of 05-OCT-2001. First hard freez on 26-OCT-2001.

Data was collected on increase plots on inventory grown or harvested in Ames in 2001.

Pathology notes were not extensive, but no exotic diseases were noted. Specific notes on Stewart's wilt and Goss's wilt were obtained. None of the exotic downy mildews were observed. The nursery was not examined for possible virus infected plants.

Environment for accessions grown in Ames during the summer of 2002. Planted in Plant Introduction fields F, D, B, NF4. Planting date was 25-APR-2002 for field F which were selected as early populations. Planting date was 07-MAY-2002 for D and B which are inbred lines. Planting date for NF4 is 00-MAY-2002 which are populations. The first pollination date was on 24-JUN-2002. First frost and freeze on the morning of 00-___-2002.

Pathology notes were not extensive, but no exotic diseases were noted. Many of the notes were taken in September which is late for earlier maturing accessions. Specific notes on Stewart's wilt and Goss's wilt were obtained when the accession was not too mature. None of the exotic downy mildews were observed. The nursery was not examined for possible virus infected plants

Pathology notes were not extensive, but no exotic diseases were noted. Many of the notes were taken in September which is late for earlier maturing accessions. Specific notes on Stewart's wilt and Goss's wilt were obtained when the accession was not too mature. None of the exotic downy mildews were observed. The nursery was not examined for possible virus infected plants.

Pathology notes were not extensive, but no exotic diseases were noted. Many of the notes were taken in September which is late for earlier maturing accessions. Specific notes on Stewart's wilt and Goss's wilt were obtained when the accession was not too mature. None of the exotic downy mildews were observed. The nursery was not examined for possible virus infected plants.

Pathology notes were not extensive, but no exotic diseases were noted. Many of the notes were taken in September which is late for earlier maturing accessions. Specific notes on Stewart's wilt and Goss's wilt were obtained when the accession was not too mature. None of the exotic downy mildews were observed. The nursery was not examined for possible virus infected plants

Environment for accessions grown in Ames during the summer of 2003 and planted early. Planted 23-APR-2003 in NCRPIS field J-6. First pollinations across all Ames environments for the summer of 2003 were performed on 27-JUN-2003.

Pathology notes were extensive. No exotic diseases were noted. Where each lot was observed, specific notes on Stewart's wilt and Goss's wilt and other diseases including virus diseases were obtained and recorded for each accession inventory. None of the exotic downy mildews were observed.

Environment for accessions grown in Ames during the summer of 2003 planted at mid planting time 1. Planted 19-MAY-2003 in NCRPIS field A. First pollinations across all Ames environments for the summer of 2003 were performed on 27-JUN-2003. Harvesting was completed 27-OCT-2003.

Pathology notes were extensive. No exotic diseases were noted. Where each lot was observed, specific notes on Stewart's wilt and Goss's wilt and other diseases including virus diseases were obtained and recorded for each accession inventory. None of the exotic downy mildews were observed.

Environment for accessions grown in Ames during the summer of 2003 planted 23-MAY-2003 in NCRPIS field J-6. First pollinations across all Ames environments for the summer of 2003 were performed on 27-JUN-2003. Harvesting was completed 27-OCT-2003.

Pathology notes were extensive. No exotic diseases were noted. Where each lot was observed, specific notes on Stewart's wilt and Goss's wilt and other diseases including virus diseases were obtained and recorded for each accession inventory. None of the exotic downy mildews were observed.

Environment for accessions grown in Ames during the summer of 2004 and planted early. Planted 15-APR-2004 in NCRPIS field W-1. First pollinations across all Ames environments for the summer of 2004 were performed on 17-JUN-2004.

Pathology notes were extensive. No exotic diseases were noted. Where each lot was observed, specific notes on Stewart's wilt and Goss's wilt and other diseases including virus diseases were obtained and recorded for each accession inventory. None of the exotic downy mildews were observed.

Environment for accessions grown in Ames during the summer of 2004 and planted mid season. Planted part 12-MAY-2004 and other 17-MAY-2004 in NCRPIS field C. First pollinations across all Ames environments for the summer of 2004 were performed on 17-JUN-2004.

Pathology notes were extensive. No exotic diseases were noted. Where each lot was observed, specific notes on Stewart's wilt and Goss's wilt and other diseases including virus diseases were obtained and recorded for each accession inventory. None of the exotic downy mildews were observed.

Environment for accessions grown in Ames during the summer of 2004 and planted mid season. Planted 12-MAY-2004 in NCRPIS field C. First pollinations across all Ames environments for the summer of 2004 were performed on 17-JUN-2004.

Pathology notes were extensive. No exotic diseases were noted. Where each lot was observed, specific notes on Stewart's wilt and Goss's wilt and other diseases including virus diseases were obtained and recorded for each accession inventory. None of the exotic downy mildews were observed.

Environment for accessions grown in Ames during the summer of 2004 and planted mid season. Planted 17-MAY-2004 in NCRPIS field C, D, W-2, W-3. First pollinations across all Ames environments for the summer of 2004 were performed on 17-JUN-2004.

Pathology notes were extensive. No exotic diseases were noted. Where each lot was observed, specific notes on Stewart's wilt and Goss's wilt and other diseases including virus diseases were obtained and recorded for each accession inventory. None of the exotic downy mildews were observed.

Environment for accessions grown in Ames during the summer of 2005. Planted 10-MAY-2005 in NCRPIS field A. First pollinations across all Ames environments for the summer of 2005 were performed on 04-JUL-2005.

Pathology notes were extensive. No exotic diseases were noted. Where each lot was observed, specific notes on Stewart's wilt and Goss's wilt and other diseases including virus diseases were obtained and recorded for each accession inventory. None of the exotic downy mildews were observed.

Environment for accessions grown in Ames during the summer of 2006. Planted 18-APR-2006 in NCRPIS field W2. First pollinations across all Ames environments for the summer of 2006 were performed on 17-JUN-2006. Pollination dates are linked to parent lot codes.

Pathology notes were extensive. No exotic diseases were noted. Where each lot was observed, specific notes on Stewart's wilt and Goss's wilt and other diseases including virus diseases were obtained and recorded for each accession inventory. None of the exotic downy mildews were observed.

Environment for accessions grown in Ames during the summer of 2006. Planted 19-MAY-2006 in NCRPIS field C, W3. First pollinations across all Ames environments for the summer of 2006 were performed on 17-JUN-2006. Pollination dates are linked to parent lot codes.

Pathology notes were extensive. No exotic diseases were noted. Where each lot was observed, specific notes on Stewart's wilt and Goss's wilt and other diseases including virus diseases were obtained and recorded for each accession inventory. None of the exotic downy mildews were observed.

Environment for accessions grown in Ames during the summer of 2006. Planted 24-APR-2006 in NCRPIS field C, W3. First pollinations across all Ames environments for the summer of 2006 were performed on 17-JUN-2006. Pollination dates are linked to parent lot codes.

Pathology notes were extensive. No exotic diseases were noted. Where each lot was observed, specific notes on Stewart's wilt and Goss's wilt and other diseases including virus diseases were obtained and recorded for each accession inventory. None of the exotic downy mildews were observed.

Environment for accessions grown in Ames during the summer of 2007. Planted between 13-MAY-2007 and 17-MAY-2007 by direct seeding in NCRPIS field N-3 and N-8 respectively. First pollinations across all Ames GEM environments for the summer of 2007 were performed on or around 08-JUL-2007.

Environment for accessions grown late in Ames during the summer of 2007. Planted between 07-JUN-2007 and 11-JUN-2007 by direct seeding in NCRPIS field N-6. Most were planted 08-JUN-2007. Days and growing degree days to silk may be unusual due to these late planting dates. First pollinations across all Ames environments for the summer of 2007 were performed on 27-JUN-2007.

Pathology notes were extensive. No exotic diseases were noted. Where each lot was observed, specific notes on Stewart's wilt and Goss's wilt and other diseases including virus diseases were obtained and recorded for each accession inventory. None of the exotic downy mildews were observed.

Environment for accessions grown in Ames during the summer of 2007. Planted 01-MAY-2007 in NCRPIS field F, A. First pollinations across all Ames environments for the summer of 2007 were performed on 27-JUN-2007. There were 42,700 plants available to plant in all summer 2007 nurseries.

Pathology notes were extensive. No exotic diseases were noted. Where each lot was observed, specific notes on Stewart's wilt and Goss's wilt and other diseases including virus diseases were obtained and recorded for each accession inventory. None of the exotic downy mildews were observed.

Environment for accessions grown in Ames during the summer of 2007. Planted 11-MAY-2007 in NCRPIS field N-6. First pollinations across all Ames environments for the summer of 2007 were performed on 27-JUN-2007.

Pathology notes were extensive. No exotic diseases were noted. Where each lot was observed, specific notes on Stewart's wilt and Goss's wilt and other diseases including virus diseases were obtained and recorded for each accession inventory. None of the exotic downy mildews were observed.

Environment for accessions grown in Ames during the summer of 2007 and transplanted. Planted between 04-MAY-2007 to 18-MAY-2007 in growth chambers and transplanted between 17-MAY-2007 and 26-JUN-2007 in NCRPIS field N-6. Days and growing degree days to silk may be unusual due to the unusual handling during establishment. First pollinations across all Ames environments for the summer of 2007 were performed on 27-JUN-2007. There were 42,700 plants available to plant in all summer 2007 nurseries.

Pathology notes were extensive. No exotic diseases were noted. Where each lot was observed, specific notes on Stewart's wilt and Goss's wilt and other diseases including virus diseases were obtained and recorded for each accession inventory. None of the exotic downy mildews were observed.

Environment for accessions grown in Ames during the summer of 2008. Planted 05-MAY-2008 in NCRPIS field C. This field was planted in 30 inch rows. On the average 40 seeds were planted per 25 ft row. Thinning was performed on 18-JUN-2008 to 25 plants per row. First pollinations across all Ames environments for the summer of 2008 were performed on 04-JUL-2008.

Pathology notes were extensive. No exotic diseases were noted. Where each lot was observed, specific notes on Stewart's wilt and Goss's wilt and other diseases including virus diseases were obtained and recorded for each accession inventory. None of the exotic downy mildews were observed.

Environment for accessions grown in Ames during the summer of 2008. Planted 06-MAY-2008 in NCRPIS fields D and W-2. First pollinations across all Ames environments for the summer of 2008 were performed on 04-JUL-2008.

Pathology notes were extensive. No exotic diseases were noted. Where each lot was observed, specific notes on Stewart's wilt and Goss's wilt and other diseases including virus diseases were obtained and recorded for each accession inventory. None of the exotic downy mildews were observed.

Environment for accessions grown in Ames during the summer of 2008. Planted 16-MAY-2008 in NCRPIS field SF-8. First pollinations across all Ames environments for the summer of 2008 were performed on 04-JUL-2008.

Pathology notes were extensive. No exotic diseases were noted. Where each lot was observed, specific notes on Stewart's wilt and Goss's wilt and other diseases including virus diseases were obtained and recorded for each accession inventory. None of the exotic downy mildews were observed.

Environment for accessions grown in Ames during the summer of 2008. Planted 17-JUN-2008 in NCRPIS field SF-8. First pollinations across all Ames environments for the summer of 2008 were performed on 04-JUL-2008.

Pathology notes were extensive. No exotic diseases were noted. Where each lot was observed, specific notes on Stewart's wilt and Goss's wilt and other diseases including virus diseases were obtained and recorded for each accession inventory. None of the exotic downy mildews were observed.

Planted by GEMs Team and turned over to the maize collection for harvest and inclusion in the collection.

Environment for accessions grown in Ames during the summer of 2009. Planted 05-31-2009 in NCRPIS field N-9. This field was planted in 30 inch rows. On the average 20 seeds were planted per 15 ft row. Thinning was performed on 06-23-2009 to 15 plants per row. First pollinations across all Ames environments for the summer of 2009 were performed on 00-000-2009. Photoperiod control planting. Shared Field with GEM group.

Pathology notes were extensive. No exotic diseases were noted. Where each lot was observed, specific notes on Stewart's wilt and Goss's wilt and other diseases including virus diseases were obtained and recorded for each accession inventory. None of the exotic downy mildews were observed.

Environment for accessions grown in Ames during the summer of 2009. Planted 22-Apr-2009 in NCRPIS field F. This field was planted in 30 inch rows. On the average 40 seeds were planted per 25 ft row. Thinning was performed on 00-000-2009 to 25 plants per row. First pollinations across all Ames environments for the summer of 2009 were performed on 26-Jun-2009.

Pathology notes were extensive. No exotic diseases were noted. Where each lot was observed, specific notes on Stewart's wilt and Goss's wilt and other diseases including virus diseases were obtained and recorded for each accession inventory. None of the exotic downy mildews were observed.

Environment for accessions grown in Ames during the summer of 2009. Planted 23-Apr-2009 in NCRPIS field A. This field was planted in 30 inch rows. On the average 40 seeds were planted per 25 ft row. Thinning was performed on 00-000-2009 to 25 plants per row. First pollinations across all Ames environments for the summer of 2009 were performed on 26-Jun-2009.

Pathology notes were extensive. No exotic diseases were noted. Where each lot was observed, specific notes on Stewart's wilt and Goss's wilt and other diseases including virus diseases were obtained and recorded for each accession inventory. None of the exotic downy mildews were observed.

Environment for accessions grown in Ames during the summer of 2009. Planted 06-05-2009 in NCRPIS field N-6. This field was planted in 30 inch rows. On the average 40 seeds were planted per 25 ft row. Thinning was performed on 00-000-2009 to 25 plants per row. First pollinations across all Ames environments for the summer of 2009 were performed on 26-Jun-2009.. Late expired PVP planting.

Pathology notes were extensive. No exotic diseases were noted. Where each lot was observed, specific notes on Stewart's wilt and Goss's wilt and other diseases including virus diseases were obtained and recorded for each accession inventory. None of the exotic downy mildews were observed.

Environment for accessions grown in Ames during the summer of 2009. Started 29-Apr-2009 and transplanted 29-Apr-2009 in NCRPIS GH3 and then to the coldframe. Planted in 10 gallon pot. First pollinations across all Ames environments for the summer of 2009 were performed on 06-26-2009.

Pathology notes were extensive. No exotic diseases were noted. Where each lot was observed, specific notes on Stewart's wilt and Goss's wilt and other diseases including virus diseases were obtained and recorded for each accession inventory. None of the exotic downy mildews were observed.

Environment for accessions grown in Ames during the summer of 2009. Transplanted 21-May-2009 in NCRPIS field W1. This field was planted in 30 inch rows. First pollinations across all Ames environments for the summer of 2009 were performed on 06-26-2009.

Pathology notes were extensive. No exotic diseases were noted. Where each lot was observed, specific notes on Stewart's wilt and Goss's wilt and other diseases including virus diseases were obtained and recorded for each accession inventory. None of the exotic downy mildews were observed.

Environment for accessions grown in Ames during the summer of 2010. Planted 04-May-2010 in NCRPIS field N-10. This field was planted in 30 inch rows. This nursery received a rain and wind storm event during the early morning hours of 18-JUL-2010 with gusts between 60-70 mph. Several accessions pollinated early were flattened and some reaching the pollination stage received green snap damage.

Environment for accessions grown in Ames during the summer of 2010. Planted 18-May-2010 in NCRPIS field N-7. This field was planted in 30 inch rows. This nursery received a rain and wind storm event during the early morning hours of 18-JUL-2010 with gusts between 60-70 mph. Several accessions pollinated early were flattened and some reaching the pollination stage received green snap damage.

Environment for accessions grown in Ames during the summer of 2010. Planted 06-May-2010 in NCRPIS field W2. This field was planted in 30 inch rows. On the average 40 seeds were planted per 25 ft row. Thinning was performed on 00-000-2010 to 25 plants per row. First pollinations across all Ames environments for the summer of 2010 were performed on 30-JUN-2010. This nursery received a rain and wind storm event during the early morning hours of 18-JUL-2010 with gusts between 60-70 mph. Several accessions pollinated early were flattened and some reaching the pollination stage received green snap damage.

Pathology notes were extensive. No exotic diseases were noted. Where each lot was observed, specific notes on Stewart's wilt and Goss's wilt and other diseases including virus diseases were obtained and recorded for each accession inventory. None of the exotic downy mildews were observed.

Environment for accessions grown in Ames during the summer of 2010. Planted 18-May-2010 in NCRPIS field SF-8 under photoperiod control. This field was planted in 30 inch rows. On the average 22 seeds were planted per 18 ft row. Thinning was performed on 00-000-2010 to 15 plants per row. First pollinations across all Ames environments for the summer of 2010 were performed on 30-JUN-2010. This nursery received a rain and wind storm event during the early morning hours of 18-JUL-2010 with gusts between 60-70 mph. Several accessions pollinated early were flattened and some reaching the pollination stage received green snap damage.

Pathology notes were extensive. No exotic diseases were noted. Where each lot was observed, specific notes on Stewart's wilt and Goss's wilt and other diseases including virus diseases were obtained and recorded for each accession inventory. None of the exotic downy mildews were observed.

Environment for accessions grown in Ames during the summer of 2010. Planted 19-May-2010 in NCRPIS field H. This field was planted in 30 inch rows. On the average 22 seeds were planted per 18 ft row. Thinning was performed on 00-000-2010 to 15 plants per row. First pollinations across all Ames environments for the summer of 2010 were performed on 30-JUN-2010. This nursery received a rain and wind storm event during the early morning hours of 18-JUL-2010 with gusts between 60-70 mph. Several accessions pollinated early were flattened and some reaching the pollination stage received green snap damage.

Pathology notes were extensive. No exotic diseases were noted. Where each lot was observed, specific notes on Stewart's wilt and Goss's wilt and other diseases including virus diseases were obtained and recorded for each accession inventory. None of the exotic downy mildews were observed.

Environment for accessions grown in Ames during the summer of 2010. Planted 20-May-2010 in NCRPIS field SF3W. This field was planted in 30 and 36 inch rows. On the average 40 seeds were planted per 25 ft row. Thinning was performed on 00-000-2010 to 25 plants per row. First pollinations across all Ames environments for the summer of 2010 were performed on 30-JUN-2010. This nursery received a rain and wind storm event during the early morning hours of 18-JUL-2010 with gusts between 60-70 mph. Several accessions root lodged and some reaching the pollination stage received green snap damage.

Pathology notes were extensive. No exotic diseases were noted. Where each lot was observed, specific notes on Stewart's wilt and Goss's wilt and other diseases including virus diseases were obtained and recorded for each accession inventory. None of the exotic downy mildews were observed.

Environment for accessions grown in Ames during the summer of 2010. Planted 17-June-2010 in NCRPIS field SF8. This field was planted in 30 inch rows. On the average 40 seeds were planted per 25 ft row. Thinning was performed on 00-000-2010 to 25 plants per row. First pollinations across all Ames environments for the summer of 2010 were performed on 30-JUN-2010. This nursery received a rain and wind storm event during the early morning hours of 18-JUL-2010 with gusts between 60-70 mph. Several accessions root lodged and some reaching the pollination stage received green snap damage.

Pathology notes were extensive. No exotic diseases were noted. Where each lot was observed, specific notes on Stewart's wilt and Goss's wilt and other diseases including virus diseases were obtained and recorded for each accession inventory. None of the exotic downy mildews were observed.

Environment for accessions grown by GEM in Ames during the summer of 2011.

Environment for accessions grown in Ames during the summer of 2011. Planted 05-May-2011 in NCRPIS field A. This field was planted in 30 inch rows. On the average 40 seeds were planted per 25 ft row. Thinning was performed on 03-Jun-2011 to 25 plants per row. First pollinations across all Ames environments for the summer of 2011 were performed on 01-JUL-2011.

Pathology notes were extensive. No exotic diseases were noted. Where each lot was observed, specific notes on Stewart's wilt and Goss's wilt and other diseases including virus diseases were obtained and recorded for each accession inventory. None of the exotic downy mildews were observed.

Environment for accessions grown in Ames during the summer of 2011. Planted 10-May-2011 in NCRPIS field W1. This field was planted in 36 inch rows. On the average 40 seeds were planted per 25 ft row. Thinning was performed on 10-Jun-2010 to 25 plants per row. First pollinations across all Ames environments for the summer of 2011 were performed on 01-JUL-2011.

Pathology notes were extensive. No exotic diseases were noted. Where each lot was observed, specific notes on Stewart's wilt and Goss's wilt and other diseases including virus diseases were obtained and recorded for each accession inventory. None of the exotic downy mildews were observed.

Environment for accessions grown in Ames during the summer of 2011. Planted 24-May-2011 in NCRPIS field W1. This field was planted in 30 inch rows. On the average 40 seeds were planted per 25 ft row. Thinning was performed on 15-Jun-2011 to 25 plants per row. First pollinations across all Ames environments for the summer of 2011 were performed on 01-JUL-2011.

Pathology notes were extensive. No exotic diseases were noted. Where each lot was observed, specific notes on Stewart's wilt and Goss's wilt and other diseases including virus diseases were obtained and recorded for each accession inventory. None of the exotic downy mildews were observed.

Environment for accessions grown by GEM in Ames during the summer of 2012. Fields N-10 was planted 10-May-2012, N-07 was planted 11-May-2012.

Environment for Hallauer populations grown by Adam Vanous under supervision of Candy Gardner and GEM during the summer of 2012. Field N-11 was planted 23-May-2012.

Environment for accessions grown in Ames during the summer of 2012. Planted 10-May-2012 in NCRPIS field SF-8. This field was planted in 30 inch rows. On the average 40 seeds were planted per 25 ft row. Thinning was performed on 13-Jun-2012 to 25 plants per row. First pollinations across all Ames environments for the summer of 2012 were performed on 29-Jun-2012.

Pathology notes were extensive. No exotic diseases were noted. Where each lot was observed, specific notes on Stewart's wilt and Goss's wilt and other diseases including a specific checkoff for virus diseases were obtained and recorded for each accession inventory. None of the exotic downy mildews were observed.

Environment for accessions grown in Ames during the summer of 2012. Planted 15-May-2012 in NCRPIS field F. This field was planted in 30 inch rows. On the average 40 seeds were planted per 25 ft row. Thinning was performed on 13-Jun-2012 to 25 plants per row. First pollinations across all Ames environments for the summer of 2012 were performed on 29-Jun-2012.

Pathology notes were extensive. No exotic diseases were noted. Where each lot was observed, specific notes on Stewart's wilt and Goss's wilt and other diseases including a specific checkoff for virus diseases were obtained and recorded for each accession inventory. None of the exotic downy mildews were observed.

Environment for popcorn grown by GEM in Ames during the summer of 2012. Field N-2 was planted on 18-May-2012.

Environment for accessions grown by GEM in Ames during the summer of 2013. Fields N-3 was planted 15-May-2013.

Environment for accessions grown by GEM in Ames during the summer of 2013. Fields N-7 was planted 08-JUN-2013.

Environment for accessions grown in Ames during the summer of 2013. Planted 15-May-2013 in NCRPIS field C. This field was planted in 36 inch rows for 20 passes and 30 inch rows for 6 passes. On the average 40 seeds were planted per 25 ft row. First emergence was on 21-May-2013. Thinning was performed on 19-Jun-2013 to 25 plants per row. First pollinations across all Ames environments for the summer of 2013 were performed on 01-Jul-2013.

Pathology notes were extensive. No exotic diseases were noted. Where each lot was observed, specific notes on Stewart's wilt and Goss's wilt and other diseases including a specific checkoff for virus diseases were obtained and recorded for each accession inventory. None of the exotic downy mildews were observed.

Environment for accessions grown in Ames during the summer of 2013. Planted 17-May-2013 in NCRPIS field N-6. This field was planted in 30 inch rows. On the average 40 seeds were planted per 25 ft row. First emergence was on 24-May-2013. Thinning was performed on 19-Jun-2013 to 25 plants per row. First pollinations across all Ames environments for the summer of 2013 were performed on 01-Jul-2013.

Pathology notes were extensive. No exotic diseases were noted. Where each lot was observed, specific notes on Stewart's wilt and Goss's wilt and other diseases including a specific checkoff for virus diseases were obtained and recorded for each accession inventory. None of the exotic downy mildews were observed.

Environment for late addition accessions grown in Ames during the summer of 2013. Started in the growth chamber 10-June-2013. Transplanted in Farm GH3 14-June-2013. Transplanted into Field C 27-June-2013.

Pathology notes were extensive. No exotic diseases were noted. Where each lot was observed, specific notes on Stewart's wilt and Goss's wilt and other diseases including a specific checkoff for virus diseases were obtained and recorded for each accession inventory. None of the exotic downy mildews were observed.

Environment for late addition accessions grown in Ames during the summer of 2013. Jab planted into Field C 18-June-2013.

Pathology notes were extensive. No exotic diseases were noted. Where each lot was observed, specific notes on Stewart's wilt and Goss's wilt and other diseases including a specific checkoff for virus diseases were obtained and recorded for each accession inventory. None of the exotic downy mildews were observed.

Environment for accessions grown by GEM in Ames during the summer of 2014. Fields N-10 was planted 08-May-2014.

Environment for accessions grown by GEM in Ames during the summer of 2014. Fields N-3 was planted 30-May-2014.

Environment for inbred accessions grown in Ames during the summer of 2014. Planted 08-May-2014 in NCRPIS field W-4. This field was planted in 30 inch rows. On the average 40 seeds were planted per 25 ft row. First emergence was on 21-May-2014. Thinning was performed on 06-Jun-2014 to 25 plants per row. First pollinations across all Ames environments for the summer of 2014 were performed on 23-Jun-2014.

Pathology notes were extensive. No exotic diseases were noted. Where each lot was observed, specific notes on Stewart's wilt and Goss's wilt and other diseases including a specific checkoff for virus diseases were obtained and recorded for each accession inventory. None of the exotic downy mildews were observed.

Environment for inbred accessions grown in Ames during the summer of 2014. Planted 07-May-2014 in NCRPIS field W-5. This field was planted in 30 inch rows. On the average 40 seeds were planted per 25 ft row. First emergence was on 21-May-2014. Thinning was performed on 09-Jun-2014 to 25 plants per row. First pollinations across all Ames environments for the summer of 2014 were performed on 23-Jun-2014.

Pathology notes were extensive. No exotic diseases were noted. Where each lot was observed, specific notes on Stewart's wilt and Goss's wilt and other diseases including a specific checkoff for virus diseases were obtained and recorded for each accession inventory. None of the exotic downy mildews were observed.

Environment for late addition accessions grown in Ames during the summer of 2014. Jab planted into Field W-4 & W-5. Multiple Planting dates.

Pathology notes were extensive. No exotic diseases were noted. Where each lot was observed, specific notes on Stewart's wilt and Goss's wilt and other diseases including a specific checkoff for virus diseases were obtained and recorded for each accession inventory. None of the exotic downy mildews were observed.

Environment for accessions grown by GEM in Ames during the summer of 2014. Fields N-6 was planted 00-May-2014.

Environment for inbred accessions grown in Ames during the summer of 2015. Planted 13-May-2015 in NCRPIS field F. This field was planted in 30 inch rows. On the average 40 seeds were planted per 25 ft row. First emergence was on 00-May-2015. Thinning was performed on 00-Jun-2015 to 25 plants per row. First pollinations across all Ames environments for the summer of 2015 were performed on 00-Jun-2015.

Pathology notes were extensive. No exotic diseases were noted. Where each lot was observed, specific notes on Stewart's wilt and Goss's wilt and other diseases including a specific checkoff for virus diseases were obtained and recorded for each accession inventory. None of the exotic downy mildews were observed.

Environment for accessions grown in Ames during the summer of 2015. Planted 19-May-2015 in NCRPIS field A. This field was planted in 30 inch rows. On the average 40 seeds were planted per 25 ft row. First emergence was on 00-May-2015. Thinning was performed on 00-Jun-2015 to 25 plants per row. First pollinations across all Ames environments for the summer of 2015 were performed on 00-Jun-2015.

Pathology notes were extensive. No exotic diseases were noted. Where each lot was observed, specific notes on Stewart's wilt and Goss's wilt and other diseases including a specific checkoff for virus diseases were obtained and recorded for each accession inventory. None of the exotic downy mildews were observed.

Environment for inbred accessions grown in Ames during the summer of 2015. Planted 13-May-2015 in NCRPIS field F. This field was planted in 30 inch rows. On the average 40 seeds were planted per 25 ft row. First emergence was on 00-May-2015. Thinning was performed on 00-Jun-2015 to 25 plants per row. First pollinations across all Ames environments for the summer of 2015 were performed on 00-Jun-2015.

Pathology notes were extensive. No exotic diseases were noted. Where each lot was observed, specific notes on Stewart's wilt and Goss's wilt and other diseases including a specific checkoff for virus diseases were obtained and recorded for each accession inventory. None of the exotic downy mildews were observed.

Environment for accessions grown by GEM in Ames during the summer of 2016. Fields N-10B was planted 04-May-2016.

Environment for inbred accessions grown in Ames during the summer of 2016. Planted 05-May-2016 in NCRPIS field D. This field was planted in 30 inch rows. On the average 40 seeds were planted per 25 ft row. First emergence was on 15-May-2016. Thinning was performed on 00-Jun-2016 to 25 plants per row. First pollinations across all Ames environments for the summer of 2016 were performed on 23-Jun-2016.

Pathology notes were extensive. No exotic diseases were noted. Where each lot was observed, specific notes on Stewart's wilt and Goss's wilt and other diseases including a specific checkoff for virus diseases were obtained and recorded for each accession inventory. None of the exotic downy mildews were observed.

Environment for inbred accessions grown in Ames during the summer of 2016. Planted 13-May-2016 in NCRPIS field W-2. This field was planted in 30 inch rows. On the average 40 seeds were planted per 25 ft row. First emergence was on 21-May-2016. Thinning was performed on 00-Jun-2016 to 25 plants per row. First pollinations across all Ames environments for the summer of 2016 were performed on 23-Jun-2016.

Pathology notes were extensive. No exotic diseases were noted. Where each lot was observed, specific notes on Stewart's wilt and Goss's wilt and other diseases including a specific checkoff for virus diseases were obtained and recorded for each accession inventory. None of the exotic downy mildews were observed.

Environment for population accessions grown in Ames during the summer of 2016. Planted 17-May-2016 in NCRPIS field SF-8. This field was planted in 30 inch rows. On the average 40 seeds were planted per 25 ft row. First emergence was on 24-May-2016. Thinning was performed on 00-Jun-2016 to 25 plants per row. First pollinations across all Ames environments for the summer of 2016 were performed on 23-Jun-2016.

Pathology notes were extensive. No exotic diseases were noted. Where each lot was observed, specific notes on Stewart's wilt and Goss's wilt and other diseases including a specific checkoff for virus diseases were obtained and recorded for each accession inventory. None of the exotic downy mildews were observed.

GEM accessions grown by the GEM team in the 2017 summer season and donated in the fall of 2017.

Environment for inbred accessions grown in Ames during the summer of 2017. Planted 07-May-2017 in NCRPIS field F. This field was planted in 30 inch rows. On the average 40 seeds were planted per 25 ft row. First emergence was on 15-May-2017. Thinning was performed on 07-Jun-2017 to 25 plants per row. First pollinations across all Ames environments for the summer of 2017 were performed on 08-Jul-2017.

Pathology notes were extensive. No exotic diseases were noted. Where each lot was observed, specific notes on Stewart's wilt and Goss's wilt and other diseases including a specific checkoff for virus diseases were obtained and recorded for each accession inventory. None of the exotic downy mildews were observed.

Environment for inbred accessions grown in Ames during the summer of 2017. Planted 16-May-2017 in NCRPIS field C. This field was planted in 30 inch rows. On the average 40 seeds were planted per 25 ft row. First emergence was on 28-May-2017. Thinning was performed on 22-Jun-2017 to 25 plants per row. First pollinations across all Ames environments for the summer of 2017 were performed on 08-Jul-2017.

Pathology notes were extensive. No exotic diseases were noted. Where each lot was observed, specific notes on Stewart's wilt and Goss's wilt and other diseases including a specific checkoff for virus diseases were obtained and recorded for each accession inventory. None of the exotic downy mildews were observed.

Environment for inbred accessions grown in Ames during the summer of 2017. Planted 02-June-2017 in NCRPIS field A. This field was planted in 30 inch rows. On the average 40 seeds were planted per 25 ft row. First emergence was on 08-June-2017. Thinning was performed on 23-Jun-2017 to 25 plants per row. First pollinations across all Ames environments for the summer of 2017 were performed on 08-Jul-2017.

Pathology notes were extensive. No exotic diseases were noted. Where each lot was observed, specific notes on Stewart's wilt and Goss's wilt and other diseases including a specific checkoff for virus diseases were obtained and recorded for each accession inventory. None of the exotic downy mildews were observed.

Environment for LH260 regeneration grown in Ames during the summer of 2017. Hand planted 26-May-2017 in NCRPIS field H. This field was not treated with pre-plant herbicide.This field was planted in 30 inch rows. On the average 40 seeds were planted per 25 ft row. First emergence was on 02-June-2017. Thinning was performed on 21-Jun-2017 to 25 plants per row. First pollinations across all Ames environments for the summer of 2017 were performed on 08-Jun-2017.

Pathology notes were extensive. No exotic diseases were noted. Where each lot was observed, specific notes on Stewart's wilt and Goss's wilt and other diseases including a specific checkoff for virus diseases were obtained and recorded for each accession inventory. None of the exotic downy mildews were observed.

Environment for tropical accessions grown in Ames during the summer of 2018. Planted 13-June-2018 in NCRPIS field A GEM Shadehouse. This field was hand planted in 30 inch rows. First pollinations performed on 00-Aug-2018. Harvest completed on 12-Oct-2018.

GEM accessions grown by the GEM team in the 2018 summer season and donated in early of 2019.

Environment for inbred accessions grown in Ames during the summer of 2018. Planted 07-May-2018 in NCRPIS field W-2. This field was planted in 30 inch rows. On the average 40 seeds were planted per 25 ft row. First emergence was on 15-May-2018. Thinning was performed on 31-May-2018 to 25 plants per row. First pollinations across all Ames environments for the summer of 2018 were performed on 25-Jun-2018. Harvest completed on 03-Oct-2018.

Pathology notes were extensive. No exotic diseases were noted. Where each lot was observed, specific notes on Stewart's wilt and Goss's wilt and other diseases including a specific checkoff for virus diseases were obtained and recorded for each accession inventory. None of the exotic downy mildews were observed.

Environment for inbred accessions grown in Ames during the summer of 2018. Planted 14-May-2018 in NCRPIS field W-2. This field was planted in 30 inch rows. On the average 40 seeds were planted per 25 ft row. First emergence was on 20-May-2018. Thinning was performed on 06-Jun-2018 to 25 plants per row. First pollinations across all Ames environments for the summer of 2018 were performed on 25-Jun-2018. Harvest completed on 19-Oct-2018.

Pathology notes were extensive. No exotic diseases were noted. Where each lot was observed, specific notes on Stewart's wilt and Goss's wilt and other diseases including a specific checkoff for virus diseases were obtained and recorded for each accession inventory. None of the exotic downy mildews were observed.

Environment for inbred accessions grown in Ames during the summer of 2018. Planted 18-May-2018 in NCRPIS field SF-8. This field was planted in 30 inch rows. On the average 40 seeds were planted per 25 ft row. First emergence was on 25-May-2018. Thinning was performed on 18-Jun-2018 to 25 plants per row. First pollinations across all Ames environments for the summer of 2018 were performed on 25-Jun-2018. Harvest completed on maize 11-Oct-2018 and Coix on 22-Oct-2018.

Pathology notes were extensive. No exotic diseases were noted. Where each lot was observed, specific notes on Stewart's wilt and Goss's wilt and other diseases including a specific checkoff for virus diseases were obtained and recorded for each accession inventory. None of the exotic downy mildews were observed.

Environment for inbred accessions grown in Ames during the summer of 2018. Planted 25-May-2018 in NCRPIS field W-2. This field was planted in 30 inch rows. On the average 40 seeds were planted per 25 ft row. First emergence was on 30-May-2018. Thinning was performed on 18-Jun-2018 to 25 plants per row. First pollinations across all Ames environments for the summer of 2018 were performed on 25-Jun-2018. Harvest completed on 15-Oct-2018.

Pathology notes were extensive. No exotic diseases were noted. Where each lot was observed, specific notes on Stewart's wilt and Goss's wilt and other diseases including a specific checkoff for virus diseases were obtained and recorded for each accession inventory. None of the exotic downy mildews were observed.

GEM accessions grown by the GEM team in the 2019 summer season and donated in the Jan-2020. Material was given to Maize Crew in ear form, without pollination bags.

Environment for population accessions grown in Ames during the summer of 2019. Planted 24-April-2019 in NCRPIS field SF-9. This field was planted in 30 inch rows. On the average 40 seeds were planted per 25 ft row. First emergence was on 14-May-2019. Thinning was performed on 20-June-2019 to 25 plants per row. First pollinations across all Ames environments for the summer of 2019 were performed on 29-Jun-2019. Last pollinations across all Ames environments for the summer 2019 were performed on 27-Aug-2019. Harvest completed on 00-Oct-2019.

Environment for population accessions grown in Ames during the summer of 2019. Planted 07-May-2019 in NCRPIS field D. This field was planted in 30 inch rows. On the average 40 seeds were planted per 25 ft row. First emergence was on 20-May-2019. Thinning was performed on 25-June-2019 to 25 plants per row. First pollinations across all Ames environments for the summer of 2019 were performed on 29-Jun-2019. Last pollinations across all Ames environments for the summer 2019 were performed on 27-Aug-2019. Harvest completed on 00-Oct-2019.

Environment for population accessions grown in Ames during the summer of 2019. Planted 04-June-2019 in NCRPIS field A-3. This field was planted in 30 inch rows. On the average 40 seeds were planted per 25 ft row. First emergence was on 10-June-2019. Thinning was performed on 00-June-2019 to 25 plants per row. First pollinations across all Ames environments for the summer of 2019 were performed on 29-Jun-2019. Last pollinations across all Ames environments for the summer 2019 were performed on 27-Aug-2019. Harvest completed on 00-Oct-2019.

Environment for population accessions grown in Ames during the summer of 2019. Planted 03-June-2019 in NCRPIS field W-5. This field was planted in 30 inch rows. On the average 40 seeds were planted per 25 ft row. First emergence was on 10-June-2019. Thinning was performed on 00-June-2019 to 25 plants per row. First pollinations across all Ames environments for the summer of 2019 were performed on 29-Jun-2019. Last pollinations across all Ames environments for the summer 2019 were performed on 27-Aug-2019. Harvest completed on 00-Oct-2019.

Environment for GEO PVP accession grown in Boone during the summer of 2019. Planted 11-June-2019 at the Burkey Farm in collaboration with the Lubberstedt Lab. 40 12.5' rows were planted with 15 seeds. First emergence was on 00-June-2019. Thinning was performed on 00-June-2019 to XX plants per row. First pollinations across all Ames environments for the summer of 2019 were performed on 00-Jun-2019. Harvest completed on 00-Oct-2019.

Environment for inbred accessions grown in Ames during the summer of 2019. Planted 08-May-2019 in growth chamber at 25-30 degrees C. Transplanted to 3 in peat pots in GH3 14-May-2019 and to Field D on 05-Jun-2019. First pollinations across all Ames environments for the summer of 2019 were performed on 29-Jun-2019. Last pollinations across all Ames environments for the summer 2019 were performed on 27-Aug-2019.

2020 Increases of GEM lines for genebank distribution. Grown in field N10.

2020 Ames Zea field increases. Planted in first planting of field SF-8 on 01-MAY-2020. First emergence 18-MAY-2020. First stand count and thinning 08-JUNE-2020. First pollinations across all Ames environments for the summer of 2020 were performed on 07-02-2021.

2020 Ames Zea field increases. Planted in second planting of field SF-8 on 01-JUNE-2020. First emergence 08-JUNE-2020. First stand count and thinning 16-JUNE-2020. First pollinations across all Ames environments for the summer of 2020 were performed on 07-02-2021.

2020 Ames Zea field increases. Planted in W-2 on 01-JUNE-2020. First emergence 08-JUNE-2020. First stand count and thinning 18-JUNE-2020.

Environment for population accessions grown in Ames during the summer of 2021. Planted 29-April-2021 in NCRPIS field A-2. This field was planted in 30 inch rows. On the average 40 seeds were planted per 25 ft row. First emergence was on 10-May-2021. Thinning was performed on 9/10-June-2021 to 25 plants per row. Field was sprayed with Impact and atrazine on 10-June-2021. First pollinations across all Ames environments for the summer of 2021 were performed on 24-Jun-2021. Last pollinations across all Ames environments for the summer 2021 were performed on 00-Aug-2021. Harvest completed on 22-Oct-2019.

Environment for population accessions grown in Ames during the summer of 2021. Planted 24-May-2021 in NCRPIS field D. This field was planted in 30 inch rows. On the average 40 seeds were planted per 25 ft row. First emergence was on 31-May-2021. Thinning was performed on 00-June-2021 to 25 plants per row. First pollinations across all Ames environments for the summer of 2021 were performed on 24-Jun-2021. Last pollinations across all Ames environments for the summer 2021 were performed on 00-Aug-2021. Harvest completed on 22-Oct-2019.

Environment for population accessions grown in Ames during the summer of 2022. Planted 17-May-2022 in NCRPIS fields SF8 and W2. These fields were planted in 30 inch rows. On the average 40 seeds were planted per 25 ft row. First emergence was on 26-May-2022. Thinning was performed on 00-June-2021 to 25 plants per row. First pollinations across all Ames environments for the summer of 2022 were performed on 07-Jun-2021. Last pollinations across all Ames environments for the summer 2022 were performed on 22-Aug-2022. Killing frost on 08-Oct-2022. Harvest completed on 00-Oct-2022.

Environment for population accessions grown in Ames during the summer of 2022. Planted 01-June-2022 in NCRPIS fields SF8 and W2. These fields were planted in 30 inch rows. On the average 40 seeds were planted per 25 ft row. First emergence was on 10-June-2022. Thinning was performed on 00-June-2021 to 25 plants per row. First pollinations across all Ames environments for the summer of 2022 were performed on 07-Jul-2022. Last pollinations across all Ames environments for the summer 2022 were performed on 22-Aug-2022. Killing frost on 08-Oct-2022. Harvest completed on 00-Oct-2022.

Environment for population accessions grown in Ames during the summer of 2022. Planted 03-June-2022 in NCRPIS fields SF8 and W2. These fields were planted in 30 inch rows. On the average 40 seeds were planted per 25 ft row. First emergence was on 10-June-2022. Thinning was performed on 00-June-2021 to 25 plants per row. First pollinations across all Ames environments for the summer of 2022 were performed on 07-Jun-2021. Last pollinations across all Ames environments for the summer 2022 were performed on 22-Aug-2022. Killing frost on 08-Oct-2022. Harvest completed on 00-Oct-2022.

Environment for population accessions grown in Ames during the summer of 2023. Planted 01-May-2023 in NCRPIS field C. This field was planted in 20 ft rows with 2 ft alleys. On the average 25 seeds were planted per 25 ft row. First emergence was on 12-May-2023. Thinning was performed on 09-June-2023 to 25 plants per row. First pollinations across all Ames environments for the summer of 2023 were performed on 20-Jun-2023. Last pollinations across all Ames environments for the summer 2023 were performed on 10-Aug-2023. First harvest across all Ames enviornments was 18-Aug-2023. Harvest completed on 10-Oct-2023.

Environment for population accessions grown in Ames during the summer of 2023. Planted 11-May-2023 in NCRPIS field C. This field was planted in 20 ft rows with 2 ft alleys. On the average 25 seeds were planted per 25 ft row. First emergence was on 19-May-2023. Thinning was performed on 20-June-2023 to 25 plants per row. First pollinations across all Ames environments for the summer of 2023 were performed on 20-Jun-2023. Last pollinations across all Ames environments for the summer 2023 were performed on 10-Aug-2023. First harvest across all Ames enviornments was 18-Aug-2023. Harvest completed on 10-Oct-2023.

Environment for population accessions grown in Ames during the summer of 2023. Planted 18-May-2023 in NCRPIS field C. This field was planted in 25ft rows with 10 ft alleys. On the average 25 seeds were planted per 25 ft row. First emergence was on 25-May-2023. Thinning was performed on 20-June-2023 to 25 plants per row. First pollinations across all Ames environments for the summer of 2023 were performed on 20-Jun-2023. Last pollinations across all Ames environments for the summer 2023 were performed on 10-Aug-2023. First harvest across all Ames enviornments was 18-Aug-2023. Harvest completed on 10-Oct-2023.

Environment for population accessions grown in Ames during the summer of 2023. Planted 23-May-2023 in NCRPIS field C. This field was planted in 25 ft rows with 10 ft alleys. On the average 25 seeds were planted per 25 ft row. First emergence was on 29-May-2023. Thinning was performed on 20-June-2023 to 25 plants per row. First pollinations across all Ames environments for the summer of 2023 were performed on 20-Jun-2023. Last pollinations across all Ames environments for the summer 2023 were performed on 10-Aug-2023. First harvest across all Ames enviornments was 18-Aug-2023. Harvest completed on 10-Oct-2023.

Burkey Farm Field B, "Row" numbers in GRIN are a combination of Range and Pass (Ex. Range 1 pass 174 = 1174) (SJA)

Environment for population accessions grown in Ames during the summer of 2024. Planted 17-MAY-2023 in NCRPIS field D. This field was planted in 15 inch rows. On the average 25 seeds were planted per 18.5 ft row. First emergence was on 23-May-2024. Thinning was performed on 10-June-2021 to 16 plants per row. At V4 plants were sprayed with a tank mix of Impact, Atrazine, Warrant, and MSO to control weeds. First pollinations across all Ames environments for the summer of 2024 were performed on 00-Jun-2021. Last pollinations across all Ames environments for the summer 2021 were performed on 00-Aug-2021. Harvest completed on 00-Oct-2019.

Environment for population accessions grown in Ames during the summer of 2024. Planted 30-MAY-2024 in NCRPIS field D. This field was planted in 15 inch rows. On the average 25 seeds were planted per 18.5 ft row. First emergence was on 04-June-2024. At V2 plants were sprayed with a tank mix of Impact, Atrazine, Warrant, and MSO to control weeds. Thinning was performed on 00-June-2021 to 16 plants per row. First pollinations across all Ames environments for the summer of 2024 were performed on 00-Jun-2021. Last pollinations across all Ames environments for the summer 2021 were performed on 00-Aug-2021. Harvest completed on 00-Oct-2019.

Original lots grown under the BGEM program in conjunction between GEM and the double haploid facility at Iowa State University.

Study Name: Maize Increase Descriptions from older Converted NC-7 data. Latitude: 42 Degrees 3 Minutes N Longitude: 93 Degrees 53 Minutes W Comment: Composite of data collected in increase plots pre 1980

Environment for accessions grown in the Ames Greenhouse and harvested in the winter of 1985-86.

Seed started in blotter boxes with lights and KNO3 solution. Seed was surfaces sterilized with 10% bleach solution before placing on blotters. Growth chamber set at 20/30 degrees celsius alternating with lights on a 12 hour on 12 off clock rotation. Approximately a week after germination, seedlings were transplanted to 3 inch peat pots and place in greenhouse. Later the peat pots were transpanted to the field.1994.

Pathology notes were not extensive, but no exotic diseases were noted. Specific notes on Stewart's wilt and Goss's wilt were obtained when the accession was not too mature. None of the exotic downy mildews were observed. The nursery was not examined for possible virus infected plants

Environment for 1996 Greenhouse regenerations. First pollination date was 3/12/1996.

Environment for 1997-98 Greenhouse regenerations. First pollination date was ...

Environment for 1998-99 Greenhouse regenerations. First pollination date was 06-JAN-1999.

Environment for 1999-2000. Greenhouse regenerations.

Environment for 2000-2001. Greenhouse regenerations.

Environment for 2006. Spring greenhouse regenerations.

Environment for 2007. Spring greenhouse regenerations. Started with cool germinator procedure.

Environment for 2008. Early greenhouse regenerations. Started 1/25/08 and later.

Environment for 2009. Late greenhouse regenerations. Planted 10/16/09.

Environment for 2010. Early greenhouse regenerations. Started 2/12/10.

Environment for 2011. Early greenhouse regenerations. Started 03/03/2011.

Overwintering teosinte, coix and corn increases.

Overwintering teosinte, coix and corn increases.

2014-15 greenhouse corn increases.

2015-16 greenhouse corn increases.

Environment for population accessions grown in Ames during the summer of 2016. Planted 06-Jun-2016 in growth chamber at 25-30 degrees C. Transplanted to 4 in peat pots in GH3 00-Jun-2016 and to12 inch pots 00-Jun-2016, one plant per pot. First pollinations across all Ames environments for the summer of 2016 were performed on 23-Jun-2016.

Environment for accessions grown in Ames during the winter of 2016-17. Planted 23-Nov-2016 in growth chamber at 25-30 degrees C. Transplanted to 4 in peat pots in GH3 00-Dec-2016 and to12 inch pots 00-Dec-2016, one plant per pot.

2019 Ames Zea GH increases grown in GH3 starting FEB-2019

Environment for accessions grown in quarantine at ISU Agronomy greenhouse room 141 during the winter of 2019-20. Planted 11-Dec-2019 in12 inch pots. First emergence 16-Dec-2019. First pollinations 00-Feb-2020.

SOP for Increase of maize seed from Thailand in the Iowa State University Agronomy Greenhouse Seed receipt and handling:

1. Seed will come via mail and be stored in Dr. Gary Munkvold’s secure, APHIS inspected facility in Seed Science. Gary’s mailing address is 160 Seed Science, 2115 Osborne Drive, Iowa State University, Ames, IA 50011. The locked, APHIS-permitted seed storage room is 121 Seed Science.

Room Preparation, Agronomy Greenhouse, Room 0141:

1. Room is cleaned in advance, ensure that doors close properly upon entry and departure.

2. Floor drains are checked to ensure proper drainage

3. Pots and soil are prepared and placed on benches in advance of planting

4. Autoclave-able garbage bags are stocked in the greenhouse room, as are all pollinating supplies.

5. Room is to be used for tropical maize increase in the months November-May only

Planting, Plant Care, Pollinating, Harvest, Insect Control, Agronomy Greenhouse, Room 0141:

1. Seeds are transferred from Dr. Gary Munkvold’s Seed Science facility to the greenhouse in a secure container.

2. Two seeds/pot are planted, remaining seeds are returned to the Munkvold facility in the secure container for storage.

3. Plants are cared for daily during growth period, technical staff observed them daily and reports any symptoms of infestation to Dr. Charles Block and to Dr. Candice Gardner.

4. Insect sticky traps are used; if white flies occur, pesticides are applied.

5. Dr. Block monitors plantings personally following emergence, and every 10-14 days during growth period. Tissues exhibiting symptoms of infestation will be testing using ELISA or PCR or traditional plating methods if disease problem is not readily diagnosed. Diseased plants will be discarded into autoclave bags and autoclaved in the head house facility, along with the pots and soil.

6. Plants are self- or sib-pollinated at flowering. Following use of tassels, tassels are to be cut off and disposed of in the autoclave bags, and autoclaved in the head house.

7. Ears are to be harvested in the greenhouse room, and dried hanging up in the same room. Following drying, they will be hand-shelled into seed envelopes and transferred to the plant pathologist’s laboratory for sampling. Following sampling, seed is to be transferred to the NCRPIS cold storage room for subsequent seed increase.

Disposal of autoclaved plant and soil debris and pots Agronomy Greenhouse Room 0101C:

1. All plant debris and soil media will either be bagged (or metal bushel baskets used for ease of handling) and autoclaved in the same manner as in #4/5. Pots can be sanitized by soaking in 5% bleach, or autoclaved.

2. Following autoclaving, waste material will be discarded in the dumpster.

2020 Ames Zea GH increases grown in GH3 starting JAN-2020.

Environment for accessions grown in quarantine at ISU Agronomy greenhouse room 141 during the winter of 2020-21. Planted 18-Nov-2020 in12 inch pots. First emergence 22-Nov-2020. On 26-Jan-2021 there was a heating malfunction in room 141 of Campus GH. Temperature reached maximum of 98.5F on 26-Jan-2021, then a maximum temperature of 102.7 was reached on 27-Jan-2021. At this point, the problem was found and corrected. Watch these pollination dates for poor viability. SOP for Increase of maize seed from Thailand in the Iowa State University Agronomy Greenhouse Seed receipt and handling: 1. Seed will come via mail and be stored in Dr. Charles Block’s secure, APHIS inspected facility in Seed Science. Charles’s mailing address is 160 Seed Science, 2115 Osborne Drive, Iowa State University, Ames, IA 50011. The locked, APHIS-permitted seed storage room is 121 Seed Science. Room Preparation, Agronomy Greenhouse, Room 0141: 1. Room is cleaned in advance, ensure that doors close properly upon entry and departure. 2. Floor drains are checked to ensure proper drainage 3. Pots and soil are prepared and placed on benches in advance of planting 4. Autoclave-able garbage bags are stocked in the greenhouse room, as are all pollinating supplies. 5. Room is to be used for tropical maize increase in the months November-March only Planting, Plant Care, Pollinating, Harvest, Insect Control, Agronomy Greenhouse, Room 0141: 1. Seeds are transferred from Dr.Charles Block’s Seed Science facility to the greenhouse in a secure container. 2. Two seeds/pot are planted, remaining seeds are returned to the Block facility in the secure container for storage. 3. Plants are cared for daily during growth period, technical staff observed them daily and reports any symptoms of infestation to Dr. Charles Block and to Dr. Candice Gardner. 4. Insect sticky traps are used; if white flies occur, pesticides are applied. 5. Dr. Block monitors plantings personally following emergence, and every 10-14 days during growth period. Tissues exhibiting symptoms of infestation will be testing using ELISA or PCR or traditional plating methods if disease problem is not readily diagnosed. Diseased plants will be discarded into autoclave bags and autoclaved in the head house facility, along with the pots and soil. 6. Plants are self- or sib-pollinated at flowering. Following use of tassels, tassels are to be cut off and disposed of in the autoclave bags, and autoclaved in the head house. 7. Ears are to be harvested in the greenhouse room, and dried hanging up in the same room. Following drying, they will be hand-shelled into seed envelopes and transferred to the plant pathologist’s laboratory for sampling. Following sampling, seed is to be transferred to the NCRPIS cold storage room for subsequent seed increase. Disposal of autoclaved plant and soil debris and pots Agronomy Greenhouse Room 0101C: 1. All plant debris and soil media will either be bagged (or metal bushel baskets used for ease of handling) and autoclaved in the same manner as in #4/5. Pots can be sanitized by soaking in 5% bleach, or autoclaved. 2. Following autoclaving, waste material will be discarded in the dumpster.

2024-2025 winter greenhouse maize increases. Started first planting (lot 01) 12-13-2024. Planted into rehydrated jiffy peat pellets in GH1. Moved to GH3 after transplanting on 12-26-2024 into 3 gal pots halfway filled with sungrow/turface/osmocote plus mix. .2nd lot direct seeded into half filled pots of the same mix on 01-22-2024. Emergence of 2nd planting on 1/30/25.3rd lot direct seeded into half filled pots of the same mix on 02-03-2025. Emergence of 3rd planting for only PI 213811 and PI 213808 on 02/10/2025. First three lots treated with Marathon 1% on 02-07-2025, using 2/3 tea for full pots of lot 01, and 1/2 tea for half pots of lots 02 and 03. Fourth lot direct seeded into half filled pots of the same mix on 3/28/2025. Emergence of 4th lot on 4/3/2025. (SJA)

Environment for increases recieved from Matthew Krakowsky done in 2006 in Tifton, Georgia and received during late summer 2006.

Environment for increases recieved from Matthew Krakowsky done in 2007 in Tifton, Georgia and received 16-Aug-2007.

Accessions grown by HSRS for increase on Kauai. Planted 08-Feb-2007.

Accessions grown by HSRS for increase on Kauai. Planted 31-Jan-2008.

Accessions grown for regeneration by Monsanto at Hawaiian Research on Molokai.

Accession increases were arranged by Dr. Randy Holley located at Northrup King in Kentucky for increase in Hawaii. The specific location used in Hawaii was not preserved in 1993-94 email to MJM. MJM reviewed computer documents on 9-Jan-2023 and did not find mention of the location of the specific nursery location. He also reviewed NK PVPs certificate applications for PVPs grown during that time and only Hawaii was mentioned. MJM also used archive digital file pdx index to search indexed maize pdf files on 9-JAN-2023. These pdfs did not include a high percentage of MJM hard copy files in 9-JAN-2-23. MJM needs to do further investigation among hard copy files as of 9-Jan-2023.

2010 Monsanto In-kind increase of 250 tropical and subtropical accessions planted in 20 kernels in 15 rows. First planting was on 26-FEB-2010 and a delay of a few rows occurred 04-MAR-2010 to mix up maturities.

2010-2011 Monsanto In-kind increase of 150 tropical and subtropical accessions planted in 20 kernels in 2 rows. Planting was near 01-NOV-2010. Material was to utilize the coolest part of the Hawaiian year. Many races especially upland were planted of materials of unknown quality and performance. Unfortunately a windstorm blew down most of the nursery and the increase was below expectations.

2011 Monsanto In-kind increase of 50 tropical and subtropical accessions for spring 2011. Shipped to Monsanto 22-Feb-2011. Regenerations were shipped back from Oahu, Hawaii on 14-Jul-2011 and was received in Ames, Iowa on 18-Jul-2011.

2011-2012 Monsanto In-kind increase of 150 tropical and subtropical accessions planted in 20/40/60/80 kernels in 2 rows. Planting was near 01-NOV-2011. Material was to utilize the coolest part of the Hawaiian year. Many races especially upland were planted of materials of unknown quality and performance. No windstorms were experienced and the increase was better than expected.

2012 Monsanto In-kind increase of 50 tropical and subtropical accessions for spring 2012. Shipped to Monsanto 06-Feb-2012. Regenerations were shipped back from Oahu, Hawaii to Williamsburg, Iowa after 14-Jul-2012. The nursery was received in Ames, Iowa from Williambsburg, Iowa on 02-Aug-2012.

2013 Monsanto In-kind increase of 50 tropical and subtropical accessions for spring 2013. Shipped to Monsanto 07-Feb-2013. Regenerations were shipped back from Oahu, Hawaii to Williamsburg, Iowa in Summer 2013. The nursery was received in Ames, Iowa from Williambsburg, Iowa on 31-Mar-2014.

2014 Monsanto In-kind increase of 100 tropical and subtropical accessions for spring 2014. Shipped to Monsanto 13-Feb-2014. Regenerations were shipped back from Oahu, Hawaii to Williamsburg, Iowa in Summer 2014. The nursery was received in Ames, Iowa from Williambsburg, Iowa on 00-000-2014.

2014 Monsanto In-kind increase of 100 tropical and subtropical accessions for spring 2015. Shipped to Monsanto 19-Feb-2015. Regenerations were shipped back from Oahu, Hawaii to Williamsburg, Iowa in Summer 2015. The nursery was received in Ames, Iowa from Williambsburg, Iowa on 00-000-2015.

Accessions grown for regeneration at the Pioneer Research Farm on the island of Kauai, Hawaii.

Accessions grown for regeneration at the Pioneer Research Farm on the island of Kauai, Hawaii.

Accessions grown for regeneration at the Pioneer Research Farm on the island of Kauai, Hawaii.

Material planted on Kauai 12-APR-2002. Harvested 31-JUL-2002. NK Agrometer registered 2502 HU between 30-APR-2002 and 31-JUL-2002. Syngenta estimated 450 HU between 12-APR-2002 and 30-APR-2002.

Material planted on Kauai ?. Harvested ?.

Accessions grown for regeneration by D & H Farms, Guthrie, Kentucky. These increases were grown in their greenhouse.

Increased in Puerto Vallarta on behalf of Matthew Krakowsky, NCState Gem coordinator.

2021 Increase grown in Puerto Vallarta, Jalisco, Mexico. Grown by contract for Matt Krakowsky with Carlos Moreno and shipped to NCRPIS for incporation into collection.

2018 Increase grown in Puerto Vallarta, Jalisco, Mexico. Grown by contract with Carlos Moreno. Includes 250 populations. Planted between 24-October-2017 and 26-October-2017.

2019 Increase grown in Puerto Vallarta, Jalisco, Mexico. Grown by contract with Carlos Moreno. Includes 125 populations. Shipping documents indicate the seed was received in Mexico on 11-October-2018 and would be planted the following week, but was returned due to a miscommunication on seed labels. The second date received in Mexico was on 23-October-2018 with an estimated planting date near 01-November-2018. Harvest completed before 08-April-2019. Received in two shipments on 17-April-2019 and 18-April-2019.

2019 Increase grown in Puerto Vallarta, Jalisco, Mexico. Grown by contract with Carlos Moreno. Includes Thailand inbred increases. Planted near 02-January-2019. Harvest completed near 06-April-2019. Ears received on 02-May-2019.

2020 Increase grown in Puerto Vallarta, Jalisco, Mexico. Grown by contract with Carlos Moreno. Includes 52 populations, with parent seed grown in US. Planted on 31-OCT-2019.

2020 Increase grown in Puerto Vallarta, Jalisco, Mexico. Grown by contract with Carlos Moreno. Includes 10 populations with parent seed grown in US. 10 populations were sent to Moreno to replace the 10 Mexico origin seed lots that were rejected at customs. Planted 13-NOV-2019.

2020-21 Increase grown in Puerto Vallarta, Jalisco, Mexico. Grown by contract with Carlos Moreno. Includes 116 populations, 2 inbreds, and 2 checks. Seed was treated with Apron XL before shipment to Moreno. Planted 10-00-2020. Received back in Ames on 00-00-2021.

2021-22 Increase grown in Puerto Vallarta, Jalisco, Mexico. Grown by contract with Carlos Moreno.

2018 Increase grown in Toluca, State of Mexico, Mexico. Grown by contract with CIMMYT. Includes 25 populations. Planted 20-April-2018.

2020 Increase grown in Toluca, State of Mexico, Mexico. Grown by contract with CIMMYT. Includes 25 populations. Was sent to CIMMYT in April 2019, but was not planted until 2020 due to shipping delays. Kept in CIMMYT cold storage until time of planting. Planted 20-MAR-2020.

Environment for increases recieved from Mark Campbell done in 2007 in Kirksville, Missouri and received 19-Dec-2007.

Environment for accessions grown at Stoneville, Mississippi.

Accessions grown under M. Krakowsky supervision at North Carolina State.

Accessions grown under M. Krakowsky supervision at North Carolina State.

Accessions grown under M. Krakowsky supervision at North Carolina State.

Accessions grown under M. Krakowsky supervision at North Carolina State.

Accessions grown under M. Krakowsky supervision at North Carolina State.

Accessions grown under M. Krakowsky supervision at North Carolina State. Received in Ames on 2-Feb-2015.

Accessions grown under M. Krakowsky supervision at North Carolina State. Received in Ames on 13-OCT-2015.

Accessions grown under M. Krakowsky supervision at North Carolina State. Received in Ames on 2-Nov-2016.

Accessions grown under M. Krakowsky supervision at North Carolina State. Received in Ames on 15-Nov-2016.

Accessions grown under M, Krakowsky supervision at North Carolina State. Received in Ames on 09-NOV-2017.

Accessions grown under J. Holland supervision at North Carolina State. Received in Ames on 09-NOV-2017.

Accessions grown under M. Krakowsky supervision at North Carolina State. Planted 05-May-2018. Harvested 04-September-2018. Received in Ames on 04-March-2019.

Evaluation for North Dakota increases grown by Frank Kutka in the summer of 2012.

Accessions grown by 3rd Millennium Genetics LLC for increase on Puerto Rico. Nursery 1234401. Planted 05-JUN-2014.

Accessions grown by 3rd Millennium Genetics LLC for increase on Puerto Rico. Nursery 1234403. Planted 09-DEC-2014.

Accessions grown by 3rd Millennium Genetics LLC for increase on Puerto Rico. Nursery 1234501. Planted 10-JUN-2015. Sent in Order 267855.

Accessions grown by 3rd Millennium Genetics LLC for increase on Puerto Rico. Nursery 1234503. Planted near 23-OCT-2015. Sent in Order 272270.

Accessions grown by 3rd Millennium Genetics LLC for increase on Puerto Rico. Nursery 1691601. Planted 08-OCT-2016. Sent in Order 284854.

Planted

Planted 20-FEB-2004.

Planted on 2000-11-21. The first date of pollination was on 2001-01-07. Bags were marked with day number 8 on that date.

Planted on 2001-11-26. The first date of pollination was on 2002-01-14 with the bag no 1.

Planted on 2002-11-25.

Planted on 27-NOV-2004. Harvested around 21-MAR-2005. 1st pollination date was on 18-JAN-2005. Shipment was received 24-MAR-2005.

Planted at ICIA in GEM winter nursery. Planted 18-NOV-2008.

Planted at ICIA in GEM winter nursery. Planted 23-NOV-2009.

Planted at ICIA in GEM winter nursery. Planted 18-NOV-2010.

Planted at ICIA in GEM winter nursery. Planted 21-NOV-2011.

Accessions grown for regeneration at Pioneer Research Farm near Salinas, Puerto Rico. Sent in order 135504 02-Feb-2001.

Accessions grown for regeneration at Pioneer Research Farm near Salinas, Puerto Rico.

Accessions grown for regeneration at Pioneer Research Farm near Salinas, Puerto Rico.

Accessions grown for regeneration at Pioneer Research Farm near Salinas, Puerto Rico.

Accessions grown for regeneration at Pioneer Research Farm near Salinas, Puerto Rico.

Planted at Isabella, Puerto Rico tropical nursery. Planted 00-MAY-2013.

Planted 12-MAR-1999. Material received from St.Croix on 09-AUG-1999.

Planted 20-APR-1999. Material received from St.Croix on 15-SEP-1999.

Planted 02-JUN-1999. Material received from St.Croix on 19-NOV-1999.

Planting damaged by hurrican Lenny.

Material received from St.Croix 2001-11-14.

Material received from St.Croix 2002-10-03.

Material received from St.Croix 2002-12-12.

Material planted on 5/3/2005.

Material planted on 5/2/2006.

Material planted on 5/15/2007.

Material planted on 5/??/2008.

Material planted on 5/06/2009.

Material received from St.Croix 2011-2012. Planted on 11/08/2011.

Material received from St.Croix 07-Nov-/2012. Planted on May 2012.

Quarantine regeneration for the first half of 1996. This nursery was planted in December, 1995.

The second quarantine regeneration for 1996. This nursery was planted on ??? ??,1996.

Material planted on after 00-May-2020. Material is not quarantine, but is tropical.

Material planted on after 00-May-2022. Material is not quarantine, but is tropical.

Material planted on after 01-May-2023. Material is not quarantine, but is tropical.

Material planted on after 01-May-2024. Material is not quarantine, but is tropical.

Material planted on 11/15?/2010. Material is not quarantine, but is tropical inbreds.

Material planted on after 14-May-2014. Material is not quarantine, but is tropical.

Material planted on after 00-May-2015. Material is not quarantine, but is tropical.

Material planted on after 00-May-2016. Material is not quarantine, but is tropical.

Material planted on after 00-May-2017. Material is not quarantine, but is tropical.

Material planted on after 00-May-2018. Material is not quarantine, but is tropical.

Material planted on 00-May-2019. Material is not quarantine, but is tropical.

Material planted on after 00-May-2021. Material is not quarantine, but is tropical.

Accessions grown for regeneration at Pioneer Research Farm near Weslaco, Texas.

Study Name: INIFAP MAIZE BANK PASSPORT RACE DATA in MEX_ALL.dbf file on 1995 LAMP CD.

The volume of 500 grams of kernels, taken on a 2000ml graduated cylinder. Measurement rounded to the nearest 10ml.

Lamp evaluation in Ames, Iowa in 1987.

Equivalent to LAMP evaluation PAIS 06, LOCALIDAD 10, ETAPA 1, from the LAMP CD 1995 PROMEDIO table.

Equivalent to LAMP evaluation PAIS 06, LOCALIDAD 30, ETAPA 1, from the LAMP CD 1995 PROMEDIO table.

Equivalent to LAMP evaluation LOCALIDAD 10, ETAPA 1, from the LAMP CD 1995 PROMEDIO table.

Equivalent to LAMP evaluation PAIS 08, LOCALIDAD 20, ETAPA 1, from the LAMP CD 1995 PROMEDIO table.

Equivalent to LAMP evaluation PAIS 08, LOCALIDAD 40, ETAPA 1, from the LAMP CD 1995 PROMEDIO table.

Equivalent to LAMP evaluation PAIS 08, LOCALIDAD 50, ETAPA 1, from the LAMP CD 1995 PROMEDIO table.

Equivalent to LAMP evaluation PAIS 08, LOCALIDAD 51, ETAPA 2, from the LAMP CD 1995 PROMEDIO table.

Equivalent to LAMP evaluation PAIS 08, LOCALIDAD 60, ETAPA 1, from the LAMP CD 1995 PROMEDIO table.

Equivalent to LAMP evaluation PAIS 08, LOCALIDAD 60, ETAPA 2, from the LAMP CD 1995 PROMEDIO table.

Equivalent to LAMP evaluation PAIS 08, LOCALIDAD 61, ETAPA 2, from the LAMP CD 1995 PROMEDIO table.

Equivalent to LAMP evaluation PAIS 08, LOCALIDAD 70, ETAPA 1, from the LAMP CD 1995 PROMEDIO table.

Equivalent to LAMP evaluation PAIS 08, LOCALIDAD 70, ETAPA 2, from the LAMP CD 1995 PROMEDIO table.

Equivalent to LAMP evaluation PAIS 08, LOCALIDAD 71, ETAPA 2, from the LAMP CD 1995 PROMEDIO table.

350 maize accessions from the NCRPIS collection were evaluated in 2000 for resistance to northern leaf blight (NLB), caused by Exserohilum turcicum race O and eyespot, caused by Kabatiella zeae. Plots were planted on 9-May-2000 at the Hinds Research farm, Ames, IA. The trial included two replicates of single rows with approximately 20-25 plants per row. Rows were spaced 0.76 m apart. Plants were inoculated on 27-JUN and 03-JUL. The first half of each row was inoculated with eyespot and the second half with northern leaf blight. Initial northern leaf blight ratings were made on 27-JUL with a second rating made between 24-AUG and 25-AUG. An average lesion length (in inches) of individual lesions was also made during the first rating date. Notes were made of any accessions with plants that expressed a chlorotic lesion response like Ht1. The score for the latter rating date was used to determine the final accession rating except for early maturing accessions where the first score was used. Each plot was given an overall score based on the average disease development on all plants in the plot.Reps were averaged. 1st northern leaf blight, 0-5 rating scale:0 = no disease;1 = lesions not present on every plant, usually five or fewer lesions per row;2 = one or two lesions per plant;3 = several lesions per plant;4 = heavy lesions, may be killing leaves;5 = several dead leaves. Second northern leaf blight, 0-9 rating scale; expansion of 0-5 scale:0 = no disease;1 = lesions not present on every plant;2 = a few lesions per plant;3 = numerous lesions per plant, but no evidence of lesion coalescence;4 = numerous lesions per leaf with some patches of leaf tissue killed below the ear;5 = whole leaves killed below the ear and some disease evident above the ear;6 = heavy disease over whole plant, some leaves above the ear severely infected;7 = heavy disease over whole plant and some leaves above the ear killed;8 = all leaf tissue above ear nearly completely diseased;9 = plants dead.

Nursery was grown at the Pioneer facilities at Johnston, Iowa and was artificially inoculated with Race O of Northern Leaf Blight (Exserhilum turcicum [Pass.] Leonard & Suggs).

Nursery was grown at the Pioneer facilities at Johnston, Iowa and was artificially inoculated with Race O of Northern Leaf Blight (Exserhilum turcicum [Pass.] Leonard & Suggs).

Nursery was grown at the Pioneer facilities at Johnston, Iowa and was artificially inoculated with Race O of Northern Leaf Blight (Exserhilum turcicum [Pass.] Leonard & Suggs). Pioneer experiment number was "DFGEM8MM5N". NCRPIS Order number was "171608,2050317".

Nursery was grown at the Pioneer facilities at Johnston, Iowa and was artificially inoculated with Race O of Northern Leaf Blight (Exserhilum turcicum [Pass.] Leonard & Suggs) and Diplodia Ear Rot (Diplodia frumenti Ellis & Everh.)

Nursery was grown at the Pioneer facilities at Johnston, Iowa and was artificially inoculated with Race O of Northern Leaf Blight (Exserhilum turcicum [Pass.] Leonard & Suggs) and Diplodia Ear Rot (Diplodia frumenti Ellis & Everh.) The NCRPIS order number was 203665. The experiment number was DR1GEMDR8N in field JHAP106.

Nursery was grown at the Pioneer facilities at Johnston, Iowa.

Nursery was grown at the Pioneer facilities at Johnston, Iowa.

Combined genotyping and phenotyping observation data for replications planted in North Carolina, Iowa, New York, and Missouri. Accessions and lots observed are documented in orders 212933, 211255, 212935, and 212934.

Accessions grown by HSRS for observation on Kauai at a highland site. Planted 08-Feb-2007.

Observations made on inventory stored in jars at various times.

223 public inbred lines were grown at the Ben Hur Plant Science Farm, Baton Rouge, Louisiana in 1992. It was planted on 23-Apr-1992 and was sprayed with Nicosulfuron at 46.2 g/ha on 21-May-1992.

Planted as a preliminary observation for a Races of the U.S. study. Planted on 2000-05-22 in field C.

Planted as a observation plot for expired PVPs for uniformity, maturity, and other appropriate descriptors. Planted 19-MAY-2006.

Planted as a observation plot for tall accessions maturity, and other appropriate descriptors were taken. Planted 06-MAY-2008.

Popcorn study

Observations extracted from PVP certificate documentation.

Study Name: CIMMYT MAIZE BANK PASSPORT RACE DATA 1995 CIMMYT-LAMP CD.

Study Name: CIMMYT MAIZE BANK PASSPORT RACE DATA

Races documented in GENESYS from data loaded into GENESYS by CIMMYT after 2024.

The observations linked to this study represent racial designations on accessions received by the NCRPIS from Dr. Major Goodman in 2004 disignated as his "Maize Racial Collection" accumulated during his career of maize studies. The designations may or may not have been verified by field observations and the geographic designation represents the address of the scientist and not the location of any field observation.

Races of maize from INTA publication: Catalogo de Recursos Geneticos de Maiz de Sudamerica, Uruguay, 1983.

Races of maize from INTA Argentina catalogue published in 1997.

Miscellanious LAMP data from unnamed LAMP data sources. Needs verification. These records will be dropped as other Methods are designated for LAMP datasets.

Races from LAMP CD 1995 PASAP table.

Experiment Type: Field . Races from LAMP CD 1995 PROMEDO table. Locality and replication not entered from the table.

Races from LAMP CD 1995 PROMEDIO table.

Races from LAMP CD 1995 PASAP table.

Races documented in Perales, H. & D. Golicher, 2014.

Review of accessions previously identified as Cusco by Wilfredo Salhuana.

Compared sweetcorns with isozymes and morphological characters.

Plants were grown in a completely randomized design in a greenhouse located on the campus of The Pennsylvania State University in University Park, PA (40°48′ N, 77°51′ W), from May through July 2009. Three biological replications were grown per accession, and replications were planted 7 d apart in the same greenhouse. Before planting, landrace seeds were soaked for 1 h in a mixture of benomyl (methyl [1-[(butylamino)carbonyl]-1H-benzimidazol-2-yl]carbamate) (Benlate fungicide, E.I. DuPont and Company, Wilmington, DE) and 1.3 M metalaxyl (2-[(2,6-dimethylphenyl)-(2-methoxy-1-oxoethyl) amino]propanoic acid methyl ester) (Allegiance fungicide, Bayer CropScience, Monheim am Rhein, Germany). Following the fungicide treatment, five seeds per accession were germinated for 48 h in darkness at 28°C in rolled germination paper (Anchor Paper Company, St. Paul, MN) moistened with 0.5 mM CaSO4, 8 mM benomyl, and 1.3 M metalaxyl. Teosinte seed was scarified with sandpaper and soaked in 0.5 mM CaSO4 for 48 h before germination. For each accession, one seedling with a 6 to 8 cm primary root was selected for each replication. The seed of each seedling was planted at a 4 cm depth although mesocotyl length varied among accessions and individuals. Plants were grown in 10.5 L pots (21 by 40.6 cm, top diameter × height, Nursery Supplies Inc., Chambersburg, PA). The growth medium was composed of 45% peat, 45% vermiculite, and 10% silica sand, limed to pH 6.0. The nutrient solution consisted of the following: 2211 μM NO3, 777 μM NH4, 398 μM CH4N2O, 411 μM P), 1858 μM K, 1455 μM Ca, 960 μM Mg, 16 μM B, 0.33 μM Cu, 7 μM Zn, 8 μM Mn, 0.85 μM Mo, and 16 μM Fe ethylenediaminetetraacetic acid. Following seedling establishment, 2 to 3 L of nutrient solution were applied as needed to each pot three to four times per week via drip irrigation using a DI-16 Dosatron fertilizer injector (Dosatron International Inc, Dallas, TX). Irrigation volume and frequency increased as plant development proceeded. Sulfuric acid was injected into the water supply to acidify the irrigation water to a pH of 6.0. Environmental data were collected hourly in the greenhouse using a HOBO U10-003 datalogger (Onset Corporation, Pocasset, MA). Mean ambient temperature was 26.5°C ± 5.9 (day) and 21.3°C ± 2.4 (night), and mean relative humidity level was 57% ± 12.2. Maximum photosynthetic flux density was 1200 μmol photons m−2 s−1.

Study Name: Corn Rootworm Resistance Screening Experiment Type: Field FIELD

Twelve seeds were planted in 1.52 m plots on 20-MAY-1994. Plots were infested on 10-JUN-1994 at a rate of 4000 eggs per meter. Roots were pulled on 01-AUG-1994. Ratings were performed on 01 and 02-AUG-1994. The Hills & Peters 1-6 rating scale was used. Check values included 7 reps of Wf9 with a mean score of 2.92, 8 reps of CI31A with a mean score of 3.17, 10 reps of KI3 with a mean score of 3.11, and 2 reps of hybrid Wf9 X W182E with a mean score of 3.20.

The susceptible check was B37 x H84 and damage to this line was 3.02 on the Hills & Peters 1-6 scale. The "resistant" check was NGSDCRW1(C4)S2 2.75 on the 1 to 6 scale. There were 10 reps for the checks, but only 1 rep for everything else in this trial. Four roots were evaluated for each rep.

Trail was in combination with GEM breeding materials.

Two hundred and twenty-two maize accessions representing popcorn germplasm from the NCRPIS collection were evaluated in 2000 for resistance to Stewart's bacterial wilt. Plots were planted on 11-May-2000 at the NCRPIS farm, Ames, IA. The trial included two replicates of single rows of approximately 15 plants per accession. Rows were spaced 0.92 m apart. Plants at the 8 to 10-leaf stage were inoculated with Erwinia stewartii on 29-JUN-2000 and 30-JUN- 2000 in the first and second replicates respectively using the pinprick method. Ten to twelve plants were inoculated per row, skipping two plants on each end. All plants were inoculated in plots where the stand count was 12 or less. Inoculum was produced from a mixture of two E. stewartii isolates, SS104 and ES9239, grown for 24 hrs on nutrient dextrose agar plates. Ratings were made between 25-AUG-2000 and 31-AUG-2000 on eight plants per row. Individual plant scores were averaged to obtain a disease rating for each plot. Scores for the two replications were averaged to obtain the overall rating for each accession. Stewart's wilt reactions were rated on a 1-9 scale where: 1 = no appreciable spread of symptoms from infection sites; lesions within 1 cm of pinpricks; 2 =limited spread from infection sites, lesions within 3 cm of pinpricks; 3 = limited spread from infection sites, predominantly toward tips of leaves; 4 = abundant lesion growth from infection sites, toward both ends of leaves; non-systemic; 5 = limited systemic infection, 15-25% of leaf area affected on inoculated leaves; 6 = Chlorosis and necrosis of 25-50% of leaf area on inoculated leaves; moderate systemic infection; 7 = Severe chlorosis and necrosis of 50-75% of leaf area on inoculated leaves; abundant systemic infection; 8 = Severe chlorosis and necrosis of 75-90% of leaf area on inoculated leaves; severe systemic infection; 9 = 90-100% infection, severe necrosis or dead plant.

Eight hundred and fifty-one maize accessions were evaluated for resistance to Stewart's wilt, caused by Pantoea (Erwinia) stewartii. Accessions were grown at the NCRPIS farm at Ames, IA in two replicate plots, with 15-18 plants per plot. The first replication was planted on 08-MAY-2002 and the second was planted on 29-MAY. Inoculations were done by the pinprick method, on 21-JUN (1st rep), 03-JUL (2nd rep), and repeated one week later for each replication. Inoculum was produced from a mixture of two P. stewartii isolates (Iowa origin). Ratings were done between 23-AUG and 06-SEP-2002, depending on maturity. Individual plant scores (10-12 plants) were averaged to obtain a disease rating for each plot. Some plots were lost due to spring flooding and only one replication was available for some accessions. A composite disease average was calculated if both replicates were available. Disease reactions were scored on a 1-9 scale where: 1= no appreciable spread of symptoms from pinpricks (within 1 cm); 2= limited spread of lesions from pinpricks (within 3 cm); 3= limited spread from pinpricks with water-soaking, chlorosis, or necrosis; spread occurring mainly toward tips of leaves; 4= abundant spread from pinpricks with water-soaking, chlorosis, or necrosis toward both ends of inoculated leaves, but no evidence of systemic infection; 5= lesions spread to the main stalk; minimal systemic infection observed; 6= moderate systemic infection with 5-25% symptomatic leaf area on non-inoculated leaves; 7= abundant systemic infection and stunting with 25-50% of the leaf area symptomatic; 8= severe systemic infection and stunting with 50-90% of the plant infected; and 9= 90-100% infection, severe necrosis or dead. The checks were B37 (mod. resistant) and A632 (mod. susc.) with average ratings of 2.6 and 5.8, respectively. Disease development was excellent and provided a good test of resistance.

Six hundred and twenty-seven maize accessions were tested for resistance to Stewart's wilt, caused by Pantoea (Erwinia) stewartii. Plots were planted on 05-May-2003 at the NCRPIS farm at Ames, IA. Accessions were grown in three replicate plots with 15-18 plants per plot (row). Inoculations were done by the pinprick method on 11-JUL-2003 at the 6 to 8-leaf stage and again on 25-JUL-2003. Inoculum was produced from a mixture of two P. stewartii isolates (Iowa origin). Ratings were done between 13-AUG-2003 and 25-SEP-2003, based on plant maturity (silks brown and dry). Individual plant scores (10-12 plants) were averaged to obtain a disease rating for each plot. Some plots were lost due to spring flooding. A composite disease average was calculated remaining plots of each accession. Disease reactions were scored on a 1-9 scale where: 1= no appreciable spread of symptoms from pinpricks (within 1 cm); 2= limited spread of lesions from pinpricks (within 3 cm); 3= limited spread from pinpricks with water-soaking, chlorosis, or necrosis; spread occurring mainly toward tips of leaves; 4= abundant spread from pinpricks with water-soaking, chlorosis, or necrosis toward both ends of inoculated leaves, but no evidence of systemic infection; 5= lesions spread to the main stalk; minimal systemic infection observed; 6= moderate systemic infection with 5-25% symptomatic leaf area on non-inoculated leaves; 7= abundant systemic infection and stunting with 25-50% of the leaf area symptomatic; 8= severe systemic infection and stunting with 50-90% of the plant infected; and 9= 90-100% infection, severe necrosis or dead. The checks were B37Ht (mod. res.), A632 (mod. susc.), and CM105 (susc.) with average ratings of 3.3, 4.3, and 4.5, respectively. The late summer drought and corn borer infestation halted disease progress, resulting in noticeably less systemic infection in the susceptible accessions.

Three hundred and seventy-three maize accessions were tested for resistance to Stewart's wilt, caused by Pantoea (Erwinia) stewartii. Plots were planted on 06-May-2004 at the NCRPIS farm at Ames, IA. Accessions were grown in three replicate plots with 15-18 plants per plot (row). Inoculations were done by the pinprick method on 08-JUN-2004 at the 6 to 8-leaf stage and again on 24-JUN-2004. Inoculum was produced from a mixture of two P. stewartii isolates (Iowa origin). Ratings were done between 29-JUL-2004 and 02-SEP-2004, based on plant maturity (silks brown and dry). Individual plant scores (10-12 plants) were averaged to obtain a disease rating for each plot. Scores for the three replications were averaged for each accession. Disease reactions were scored on a 1-9 scale where: 1= no appreciable spread of symptoms from pinpricks (within 1 cm); 2= limited spread of lesions from pinpricks (within 3 cm); 3= limited spread from pinpricks with water-soaking, chlorosis, or necrosis; spread occurring mainly toward tips of leaves; 4= abundant spread from pinpricks with water-soaking, chlorosis, or necrosis toward both ends of inoculated leaves, but no evidence of systemic infection; 5= lesions spread to the main stalk; minimal systemic infection observed; 6= moderate systemic infection with 5-25% symptomatic leaf area on non-inoculated leaves; 7= abundant systemic infection and stunting with 25-50% of the leaf area symptomatic; 8= severe systemic infection and stunting with 50-90% of the plant infected; and 9= 90-100% infection, severe necrosis or dead. The checks were B37 (mod. resistant) and A632 (mod. susc.) with average ratings of 3.1 and 4.8, respectively. Disease development was slowed by the cooler than normal summer. The susceptible accessions seemed to rate slightly lower than normal (less systemic infection) while the resistant accessions seemed to respond normally.

Three hundred and eighty-four maize accessions were tested for resistance to Stewart's wilt, caused by Pantoea (Erwinia) stewartii. Plots were planted on 20-May-2005 at the NCRPIS farm at Ames, IA. Accessions were grown in three replications with 15-18 plants per plot. Two inoculations were done using the pinprick method, with one week between inoculations. The early maturing entries were inoculated on 17-JUN (6 to 8-leaf) and on 24-JUN. The rest of the entries were inoculated on 24-JUN and 30-JUN. Inoculum was grown from a mixture of two P. stewartii isolates of Iowa origin. Ratings were done by maturity (>50% of plants in plot with brown, dry silks) and took place from 21-JUL to 06-SEP. Individual scores (10-12 plants) were averaged to obtain plot ratings. Scores for the three plots were averaged for each accession. Disease reactions were scored on a 1-9 scale where: 1= no appreciable spread of symptoms from pinpricks (within 1 cm); 2= limited spread of lesions from pinpricks (within 3 cm); 3= limited spread from pinpricks with water-soaking, chlorosis, or necrosis; spread occurring mainly toward tips of leaves; 4= abundant spread from pinpricks with water-soaking, chlorosis, or necrosis toward both ends of inoculated leaves, but no evidence of systemic infection; 5= lesions spread to the main stalk; minimal systemic infection observed; 6= moderate systemic infection with 5-25% symptomatic leaf area on non-inoculated leaves; 7= abundant systemic infection and stunting with 25-50% of the leaf area symptomatic; 8= severe systemic infection and stunting with 50-90% of the plant infected; and 9= 90-100% infection, severe necrosis or dead. Six inbreds were used as checks, Mo21R (resist.), B37 Ht (mod. res.), A629 (mid-check), A632 Ht (mod. susc.), Mt42 (mod. susc.) and C42 (very susc.). Disease development was nearly optimal with good separation of resistance.

Five hundred and twelve US inbred maize accessions were tested for resistance to Stewart's wilt caused by Pantoea (Erwinia) stewartii. Plots were planted on 23-May-2006 at the NCRPIS farm at Ames, IA. Two single-row plots were planted per entry, with 15-18 plants per row. Inoculations were done by the pinprick method on 22-JUN at the 6 to 8-leaf stage and again on 29-JUN-2006. Inoculum was produced from a mixture of two P. stewartii isolates (Iowa origin). Disease ratings (one time per plot) were done between 02-AUG-2006 and 02-SEP-2006, based on plant maturity (>50% dry silks). Individual plant scores (10-12 plants) were averaged to obtain a rating for each plot and the two plots were averaged. Disease reactions were scored on a 1-9 scale where: 1= no appreciable spread of symptoms from pinpricks (within 1 cm); 2= limited spread of lesions from pinpricks (within 3 cm); 3= limited spread from pinpricks with water-soaking, chlorosis, or necrosis; spread occurring mainly toward tips of leaves; 4= abundant spread from pinpricks with water-soaking, chlorosis, or necrosis toward both ends of inoculated leaves, but no evidence of systemic infection; 5= minimal systemic infection observed; 6= moderate systemic infection with 5-25% symptomatic leaf area on non-inoculated leaves; 7= abundant systemic infection with 25-50% of the leaf area symptomatic; 8= severe systemic infection with 50-90% of the plant infected; and 9= 90-100% infection, severe necrosis or dead. Six inbred checks were used: Mo21R (resist.) with an average rating of 1.1, B37Ht (mod. res.), with an average rating of 3.6, A629 (mid-check) with an average rating of 4.9, A632Ht (mod. susc.) with an average rating of 5.5, Mt42 (mod. susc.) with an average rating of 6.1 and C42 (highly susc.) with an average rating of 8.2. Disease pressure was higher in 2006 than in most years with the presence of considerable natural infection.

One hundred and twenty-three accessions were tested for resistance to Stewart's wilt caused by Pantoea (Erwinia) stewartii. Plots were planted on 05-June-2007 at the NCRPIS farm at Ames, IA. Three single-row plots were planted per entry with 16 plants per row. Inoculations were done by the pinprick method on 28-JUN-2007 at the 6-leaf stage and again on 05-JUL-2007 at the 8-leaf stage. Inoculum was produced from a mixture of two P. stewartii isolates (Iowa origin). Disease ratings were done between 20-AUG-2007 and 09-SEP-2007, and were based on plant maturity (>50% dry silks). Individual plant scores (10-12 plants per plot) to obtain a rating for each plot and the three reps were averaged. Disease reactions were scored on a 1-9 scale where:1= no appreciable spread of symptoms from pinpricks (within 1 cm); 2= limited spread of lesions from pinpricks (within 3 cm); 3= limited spread from pinpricks with water-soaking, chlorosis, or necrosis; spread occurring mainly toward tips of leaves; 4= abundant spread from pinpricks toward both ends of inoculated leaves, but no evidence of systemic infection; 5= minimal systemic infection observed; 6= moderate systemic infection with 5-25% symptomatic leaf area on non-inoculated leaves; 7= abundant systemic infection with 25-50% of the leaf area symptomatic; 8= severe systemic infection with 50-90% of the plant infected; and 9= 90-100% infection, severe necrosis or dead. Six inbred checks were used, Mo21R (resist.) with an average rating of 1.0, B37 Ht (mod. res.) with an average rating of 3.0, A629 (mid-check) with an average rating of 3.7, A632 Ht (mod. susc.) with an average rating of 6.0, Mt42 (mod. susc.) with an average rating of 9.0 and C42 (very susc.) with an average rating of 9.0. All checks performed as expected except Mt42, which was more susceptible than normal. Disease developed very rapidly after the first inoculation, but the weather soon became hot and dry which seemed to slow further development.

Two hundred ninety-eight accessions were tested for resistance to Stewart's wilt, caused by Pantoea (Erwinia) stewartii. Plots were planted on 21-May-2009 at Ames, IA. Three single-row plots were planted per entry and thinned to 16 plants per row. Inoculations were done by the pinprick method on 01-JUL at the 6-leaf stage and on 08-JUL at the 8-leaf stage. Inoculum was produced from a mixture of three isolates, ES9211, ES9239 and ES9245 (Iowa origin). Disease ratings were done from 13-AUG to 31-AUG. Ten to 12 plants were rated per plot and an average rating was calculated for each plot. Disease reactions were scored on a 1-9 scale where: 1= no appreciable spread of symptoms from pinpricks (less than 1 cm); 2= limited spread of lesions from pinpricks (1 to 3 cm); 3= limited spread from pinpricks with water-soaking, chlorosis, or necrosis; lesions spreading mainly toward tips of leaves; 4= abundant spread of lesions in both directions on inoculated leaves, with water-soaking, chlorosis, or necrosis, but no evidence of systemic infection; 5= lesions spread to the leaf base, reaching the stalk; minimal systemic infection observed; 6= moderate systemic infection with 5-25% symptomatic leaf area on non-inoculated leaves; 7= abundant systemic infection and stunting with 25-50% of the leaf area affected; 8= severe systemic infection and stunting with 50-90% of the plant infected; 9= 90-100% infection, severe necrosis or dead. Plants with 1, 2 (highly resistant) and 3 (resistant) ratings have definite resistance. Plants with a 4,5,6 ratings are moderately susceptible and plants with a 7,8,9 ratings are highly susceptible. Six inbreds were used as checks, Mo21R (resist.) which had an average rating of 1.0, B37 Ht (mod. res.), with an average rating of 3.3, A629 (mid-check) with an average rating of 4.0, A632 Ht (mod. susc.) with an average rating of 5.1, Mt42 (very susc.) with an average rating of 7.0 and C42 (highly susc.) with an average rating of 8.1.

Two hundred fifty-nine accessions were tested for resistance to Stewart's wilt caused by Pantoea (Erwinia) stewartii. Plots were planted on 28-May-2010 at Ames, IA. Three replications of single-row plots were planted per entry. Inoculations were done by the pinprick method on 06-JUL at the 7 to 9-leaf stage and on 13-JUL at the 10 to 11-leaf stage. Inoculum was produced from a mixture of three isolates, ES9211, ES9239 and ES9245 (Iowa origin). Disease ratings were done from 18-AUG to 02-SEP. Ten to 12 plants were rated per plot and an average rating was calculated for each plot. Disease reactions were scored on a 1-9 scale where: 1= no appreciable spread of symptoms from pinpricks (less than 1 cm); 2= limited spread of lesions from pinpricks (1 to 3 cm); 3= limited spread from pinpricks with water-soaking, chlorosis, or necrosis; lesions spreading mainly toward tips of leaves; 4= abundant spread of lesions in both directions on inoculated leaves, with water-soaking, chlorosis, or necrosis, but no evidence of systemic infection; 5= lesions spread to the leaf base, reaching the stalk; minimal systemic infection observed; 6= moderate systemic infection with 5-25% symptomatic leaf area on non-inoculated leaves; 7= abundant systemic infection and stunting with 25-50% of the leaf area affected; 8= severe systemic infection and stunting with 50-90% of the plant infected; 9= 90-100% infection, severe necrosis or dead. Accessions with 1, 2 (highly resistant) and 3 (resistant) ratings have definite resistance. Accessions with a 4,5,6 ratings are moderately susceptible and accessions with 7,8,9 ratings are highly susceptible. Six inbreds were used as checks, Mo21R (resist.) which had an average rating of 1.0, B37 Ht (mod. res.), with an average rating of 3.1, A629 (mid-check) with an average rating of 4.1, A632 Ht (mod. susc.) with an average rating of 6.4, Mt42 (very susc.) with an average rating of 8.9 and C42 (highly susc.) with an average rating of 9.0.

One hundred sixty-three maize accessions were tested for resistance to Stewart's wilt caused by Pantoea (Erwinia) stewartii. Plots were planted on 19-May-2011 at Ames, IA. Three single-row plots were planted per entry. Inoculations were done by the pinprick method on 05-JUL-2011 at the 6 to 8-leaf stage and on 13-JUL at the 8 to 10-leaf stage. Inoculum was produced from a mixture of two isolates, ES9239 and ES9245 (Iowa origin). Disease ratings were done from 18-AUG to 31-AUG. Ten to 12 plants were rated per plot and an average rating was calculated for each plot. Disease reactions were scored on a 1-9 scale where: 1= no appreciable spread of symptoms from pinpricks (less than 1 cm); 2= limited spread of lesions from pinpricks (1 to 3 cm); 3= lesions spreading from the point of inoculation mainly toward tips of leaves with water-soaking, chlorosis, and necrosis; 4= abundant spread of lesions in both directions on inoculated leaves, with water-soaking, chlorosis, and necrosis, but no evidence of systemic infection; 5= lesions spread to the leaf base and reach the stalk; minimal systemic infection observed; 6= moderate systemic infection with 5-25% symptomatic leaf area on non-inoculated leaves; 7= abundant systemic infection and stunting with 25-50% of the leaf area affected; 8= severe systemic infection and stunting with 50-90% of the plant infected; 9= 90-100% infection, severe necrosis or dead. Accessions with 1, 2 (highly resistant) and 3 (resistant) ratings have definite resistance. Accessions with 4,5,6 ratings are moderately susceptible and accessions with 7,8,9 ratings are highly susceptible. Six inbreds were used as checks, Mo21R (resist.) which had an average rating of 1.0, B37 Ht (mod. res.), with an average rating of 3.1, A629 (mid-check) with an average rating of 4.0, A632 Ht (mod. susc.) with an average rating of 6.5, Mt42 (very susc.) with an average rating of 8.8 and C42 (highly susc.) with an average rating of 8.8.

Two hundred and fifty maize accessions were tested for resistance to Stewart's wilt caused by Pantoea (Erwinia) stewartii. Plots were planted on 24-May-2012 at Ames, IA. Three single-row plots were planted per entry and thinned to 16 plants per row. Inoculations were done by the pinprick method on 27-JUN at the 6 to 8-leaf stage and on 03-JUL at the 8 to 10-leaf stage. Inoculum was produced from a mixture of three isolates, ES9211, ES9239 and ES9245 (Iowa origin). Disease ratings were done from 15-AUG to 31-AUG. Ten to 12 plants were rated per plot and an average rating was calculated for each plot. Disease reactions were scored on a 1-9 scale where: 1= no appreciable spread of symptoms from pinpricks (less than 1 cm); 2= limited spread of lesions from pinpricks (1 to 3 cm); 3= limited spread from pinpricks with water-soaking, chlorosis, or necrosis; lesions spreading mainly toward tips of leaves; 4= abundant spread of lesions in both directions on inoculated leaves, with water-soaking, chlorosis, or necrosis, but no evidence of systemic infection; 5= lesions spread to the leaf base, reaching the stalk; minimal systemic infection observed; 6= moderate systemic infection with 5-25% symptomatic leaf area on non-inoculated leaves; 7= abundant systemic infection and stunting with 25-50% of the leaf area affected; 8= severe systemic infection and stunting with 50-90% of the plant infected; 9= 90-100% infection, severe necrosis or dead. Plants with 1,2 (highly resistant) and 3 (resistant) ratings have good resistance. Plants with a 4,5,6 ratings are moderately susceptible and plants with a 7,8,9 ratings are highly susceptible. Six inbreds were used as checks, Mo21R (resist.) which had an average rating of 1.0, B37 Ht (mod. res.), with an average rating of 3.2, A629 (mid-check) with an average rating of 4.1, A632 Ht (mod. susc.) with an average rating of 6.3, Mt42 (very susc.) with an average rating of 9.0 and C42 (highly susc.) with an average rating of 8.9.

Two hundred and eight maize accessions were tested for resistance to Stewart's wilt caused by Pantoea (Erwinia) stewartii. Plots were planted on 08-June-2013 at Ames, IA. Three replications of single-row plots were planted per entry and thinned to 16 plants per row. Inoculations were done by the pinprick method on 10-JUL at the 6 to 8-leaf stage and on 16-JUL at the 8 to 10-leaf stage. Inoculum was produced from a mixture of three isolates, ES9239, ES9245, and ES9264 (Iowa origin). Disease ratings were done from 26-AUG to 31-AUG. Ten to 12 plants were rated per plot and an average rating was calculated for each plot. Disease reactions were scored on a 1-9 scale where: 1= no appreciable spread of symptoms from pinpricks (less than 1 cm); 2= limited spread of lesions from pinpricks (1 to 3 cm); 3= lesions spreading from the point of inoculation mainly toward tips of leaves with water-soaking, chlorosis, and necrosis; 4= abundant spread of lesions in both directions on inoculated leaves, with water-soaking, chlorosis, and necrosis, but no evidence of systemic infection; 5= lesions spread to the leaf base and reach the stalk; minimal systemic infection observed; 6= moderate systemic infection with 5-25% symptomatic leaf area on non-inoculated leaves; 7= abundant systemic infection and stunting with 25-50% of the leaf area affected; 8= severe systemic infection and stunting with 50-90% of the plant infected; 9= 90-100% infection, severe necrosis or dead. Accessions with 1, 2 (highly resistant) and 3 (resistant) ratings have definite resistance. Accessions with 4,5,6 ratings are moderately susceptible and accessions with 7,8,9 ratings are highly susceptible. Seven inbreds were used as checks, WIL500 (highly resist.) with an average rating of 1.0, Mo21R (resist.) with an average rating of 1.6, B37 Ht (mod. res.), with an average rating of 2.7, A629 (mid-check) with an average rating of 4.0, A632 Ht (mod. susc.) with an average rating of 6.5, Mt42 (very susc.) with an average rating of 8.0 and C42 (highly susc.) with an average rating of 8.4.

One hundred and sixty maize accessions were tested in 2014 for resistance to Stewart's wilt caused by Pantoea (Erwinia) stewartii. Plots were planted on 11-June-2014 at Ames, IA. Three replications of single-row plots were planted per entry and thinned to 16 plants per row. Inoculations were done by the pinprick method on 15-JUL at the 6 to 8-leaf stage and on 23-JUL at the 8 to 10-leaf stage. Inoculum was produced from a mixture of three isolates, ES9211, ES9239, and ES9245 (Iowa origin). Disease ratings were done from 05-SEP to 09-SEP-2014. Ten to 12 plants were rated per plot and an average rating was calculated for each plot. Disease reactions were scored on a 1-9 scale where: 1= no appreciable spread of symptoms from pinpricks (less than 1 cm); 2= limited spread of lesions from pinpricks (1 to 3 cm); 3= lesions spreading from the point of inoculation mainly toward tips of leaves with water-soaking, chlorosis, and necrosis; 4= abundant spread of lesions in both directions on inoculated leaves, with water-soaking, chlorosis, and necrosis, but no evidence of systemic infection; 5= lesions spread to the leaf base and reach the stalk; minimal systemic infection observed; 6= moderate systemic infection with 5-25% symptomatic leaf area on non-inoculated leaves; 7= abundant systemic infection and stunting with 25-50% of the leaf area affected; 8= severe systemic infection and stunting with 50-90% of the plant infected; 9= 90-100% infection, severe necrosis or dead. Accessions with 1, 2 (highly resistant) and 3 (resistant) ratings have definite resistance. Accessions with 4,5,6 ratings are moderately susceptible and accessions with 7,8,9 ratings are highly susceptible. Six inbreds were used as checks, WIL500 (highly resist.) with an average rating of 1.0, Mo21R (resist.) with an average rating of 1.7, B37 (mod. res.), with an average rating of 3.5, A629 (mid-check) with an average rating of 3.8, A632 Ht (mod. susc.) with an average rating of 5.6, and Mt42 (very susc.) with an average rating of 8.9.

2000 maize accessions were obtained from the 14,000 accessions in the NCRPIS collection in 1997. Accessions were selected by the maize curator (M. Millard) as representative of sweet and field corn germplasm from the U.S. and maize germplasm from other regions of the world adapted to temperate climates. Planting was on 22-May-1997 at the U. of Illinois South Farm, Urbana, Illinois. The trial included two replicates of single rows of approximately 15 plants per accession. Rows were spaced 0.76 m apart. Plant stands were adequate for all but 9 accessions. Plants at the 3 to 6-leaf stage were inoculated with E. stewartii on 25-JUN-1997 and 30-JUN-1997 in the first and second replicates respectively using the pinprick method. Inoculum was produced as described previously from a mixture of isolates including those collected in May 1997 from naturally infected plants in southern Illinois and those isolated from trials in Illinois in 1996 and maintained over winter in the greenhouse and at -80 degrees C. Reactions to E. stewartii were rated on a 1-9 scale where 1=no appreciable spread of symptoms from pinpricks; 2=limited spread with water-soaking, chlorosis, or necrosis within 3 cm of pinpricks; 3=limited spread from pinpricks with water-soaking, chlorosis, or necrosis predominantly toward tips of leaves; 4=abundant spread from pinpricks with water-soaking, chlorosis, or necrosis toward both ends of inoculated leaves, but no symptoms on noninoculated leaves; 5=minimal systemic infection observed as small, linear streaks of symptoms on noninoculated leaves; 6=moderate systemic infection with 5-25% symptomatic leaf area of noninoculated leaves and minimal stunting; 7=abundant systemic infection and stunting with 25-50% of the leaf area symptomatic; 8=severe systemic infection and stunting with 50-90% of the plant infected; and 9=90-100% infection, severe necrosis, or a dead plants. 0n 8-11 July and 16-17 ratings were performed. Checks 'Miracle'=1.9, 'Jubilee'=5.9.

Observations extracted from the Utility Patent documentation.

Seeds are rinsed in 10% bleach and placed on blotters in deep plastic boxes. Separation of seed is 2 cm or more. Fungicides are applied when needed. Boxes are placed in germinator set on alternating 20-30 degrees C and alternating light-dark. When seedling leaves begin emerging from the coleoptile, they are transplanted to 7.6 cm jiffy pots. Soil is added to the pots as the seedlings develop. These jiffy pots are transplanted to large plastic pots when the seedlings are fully established.

Seeds are surface sterilized using a 10% bleach solution. Germination boxes, forceps, spoon, and any other utensils are surfaced sterilized in 50% bleach solution and rinsed with distilled water. Blotters are wetted with distilled water and sterilized in microwave for 1 minute. Three blotters are placed inside single germination box. Seeds are placed on blotter separated by at least 2 cm. Activated carbon is sprinkled on top of kernel, completely covering a single kernel at a time. Boxes are placed in germinator set on alternating 20-30 degrees C and alternating light-dark. When seedling leaves begin emerging from the coleoptile, they are transplanted to 7.6 cm jiffy pots half filled with wetted soil and placed inside a tray. Water is added to tray within 24 hours of seedlings drying out. Soil is added to the pots as the seedlings develop. When seedlings are developed and roots have emerged jiffy pots are transplanted to 3/4 filled large plastic pots. All soil mixture is 50% potting mix, 25% field soil, and 25% sand.

Germinations are performed in a high humidity germinator set at 12 hours 20 degrees centigrade in the dark alternating with 12 hours 30 degrees centigrade in the light. 50 seeds are wrapped in 2 paper towels for each replication. Replications are rubber banded together. 10-20 accessions are placed in plastic tubs. Towels are kept moist during germination period. Evaluations are performed at 7 and 10 days, or as needed.