Methods
Multiple harvests were combined by Seed Storage to make this 'AVAIL' lot.
1961 Brassica Regeneration
Study Name: 1966 Brassica Regeneration: Location: PI FARM; Latitude: 42 Degrees 0 Minutes N; Longitude: 93 Degrees 30 Minutes W ; Year harvested: 1966
Study Name: 1967 Brassica Regeneration: Location: PI FARM; Latitude: 42 Degrees 0 Minutes N; Longitude: 93 Degrees 30 Minutes W ; Year harvested: 1967
Study Name: 1979 Brassica Regeneration: Location: PI FARM; Latitude: 42 Degrees 0 Minutes N; Longitude: 93 Degrees 30 Minutes W ; Year harvested: 1979
Study Name: 1980 Brassica Regeneration: Location: PI FARM; Latitude: 42 Degrees 0 Minutes N; Longitude: 93 Degrees 30 Minutes W ; Year harvested: 1980
Study Name: 1984 Brassica Regeneration: Location: PI FARM; Latitude: 42 Degrees 0 Minutes N; Longitude: 93 Degrees 30 Minutes W ; Year harvested: 1984
Study Name: 1993 Brassica Regeneration: Location: PI FARM; Latitude: 42 Degrees 0 Minutes N; Longitude: 93 Degrees 30 Minutes W ; Year harvested: 1993
Study Name: 1994 Brassica Regeneration: Location: PI FARM; Latitude: 42 Degrees 0 Minutes N; Longitude: 93 Degrees 30 Minutes W ; Year harvested: 1994
Study Name: 1995 Brassica Regeneration: Location: PI FARM; Latitude: 42 Degrees 0 Minutes N; Longitude: 93 Degrees 30 Minutes W ; Year harvested: 1995
Study Name: 1996 Brassica Regeneration: Location: PI FARM; Latitude: 42 Degrees 0 Minutes N; Longitude: 93 Degrees 30 Minutes W ; Year harvested: 1996
Study Name: 1997 Brassica Regeneration: Location: PI FARM; Latitude: 42 Degrees 0 Minutes N; Longitude: 93 Degrees 30 Minutes W ; Year harvested: 1997
Study Name: 1998 Brassica Regeneration: Location: PI FARM; Latitude: 42 Degrees 0 Minutes N; Longitude: 93 Degrees 30 Minutes W ; Year harvested: 1998
Study Name: 1999 Brassica Regeneration: Location: PI FARM; Latitude: 42 Degrees 0 Minutes N; Longitude: 93 Degrees 30 Minutes W ; Year harvested: 1999
Study Name: 2000 Brassica Regeneration: Location: PI FARM; Latitude: 42 Degrees 0 Minutes N; Longitude: 93 Degrees 30 Minutes W ; Year harvested: 2000
Study Name: 2001 Brassica Regeneration: Location: PI FARM; Latitude: 42 Degrees 0 Minutes N; Longitude: 93 Degrees 30 Minutes W ; Year harvested: 2001
Study Name: 2002 Brassica Regeneration: Location: PI FARM; Latitude: 42 Degrees 0 Minutes N; Longitude: 93 Degrees 30 Minutes W ; Year harvested: 2002
Study Name: 2003 Brassica Regeneration: Location: PI FARM; Latitude: 42 Degrees 0 Minutes N; Longitude: 93 Degrees 30 Minutes W ; Year harvested: 2003
Study Name: 2004 Brassica Regeneration. Location: PI FARM; Latitude: 42 Degrees 0 Minutes N; Longitude: 93 Degress 30 Minutes W; Year harvested: 2004
Study Name: 2005 Brassica Regeneration. Location: PI FARM; Latitude: 42 Degrees 0 Minutes N; Longitude: 93 Degress 30 Minutes W; Year harvested: 2005
Study Name: 2006 Brassica Regeneration. Location: PI FARM; Latitude: 42 Degrees 0 Minutes N; Longitude: 93 Degress 30 Minutes W; Year harvested: 2006
Study Name: 2007 Brassica Regeneration. Location: PI FARM; Latitude: 42 Degrees 0 Minutes N; Longitude: 93 Degress 30 Minutes W; Year harvested: 2007
2008 Brassicaceae Regenerations - Laura Marek
Camelina increases from NE9
Study Name: Brassica Descriptors Obtained During Collection Maintenance Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year harvested: 1990
Study Name: Brassica Descriptors Obtained During Collection Maintenance Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year harvested: 1992
Study Name: Brassica Descriptors Obtained During Collection Maintenance Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year harvested: 1993
Study Name: Brassica Descriptors Obtained During Collection Maintenance Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year harvested: 1994
Study Name: Brassica Descriptors Obtained During Collection Maintenance Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year harvested: 1995
Study Name: Brassica Descriptors Obtained During Collection Maintenance Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year harvested: 1996
Study Name: Brassica Descriptors Obtained During Collection Maintenance Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year harvested: 1997
Study Name: Brassica Descriptors Obtained During Collection Maintenance Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year harvested: 1998
Study Name: Brassica Descriptors Obtained During Collection Maintenance Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year harvested: 1998
Study Name: Brassica Descriptors Obtained During Collection Maintenance ; Location: PI FARM; Latitude: 42 Degrees 0 Minutes N; Longitude: 93 Degrees 30 Minutes W; Year harvested: 2000
Study Name: Brassica Descriptors Obtained During Collection Maintenance ; Location: PI FARM; Latitude: 42 Degrees 0 Minutes N; Longitude: 93 Degrees 30 Minutes W; Year harvested: 2001
Study Name: 2006 Brassica juncea & B. carinata Taxonomy Study
Original seed that is 'AVAIL' for distribution
An increase lot that was received from a site other than PI locations.
Digital photo, full plant in field, NCRPIS.
Scanned image of fresh plant stem with siliques & flowers
Scan of a sample of harvested/collected seeds, 200 dpi scan (3200 x 2400 pixels)
Scan of a sample of fresh siliques @ 200 dpi scan (3200 x 2400 dpi)
Seed colors for the distribution lots of the Brassica collection were scored using the R.H.S. Colour Chart. The scored colors were than modified to fit the 1-9 rating seed color scale
Fatty acid percentages of oil in seeds were determined on an Agilent 689ON GC equipped with a flame-ionization detector and an autosampler injector after converting the fatty acids to methyl esters. 50 seeds per accession were ground in 5 mL of 0.25 M sodium methoxide solution in 20 mL scintillation vials with a FineMech homogenizer for 1 min or until no whole seeds were present. Vials were sealed with aluminum lined screw caps and placed in a 60oC aluminum heating block for 30 min. After removing the vials from the block, 5 mL each of hexane and saturated sodium chloride were added and the contents thoroughly mixed. After the layers separated, 0.5mL aliquots from the top hexane layers were placed into 2 mL GC target vials, diluted to 2 mL with hexane and sealed with crimp caps. The vials were placed in the GC autosampler and injected onto the capillary column of a Hewlett Packard 5890 Series II gas chromatograph (GC) equipped with a flame-ionization detector and a 7963 autosampler/injector. GC separations were obtained on a SP-2380 30m X 0.25mm i.d. poly (90% biscyanopropyl/10% cyanopropylphenyl siloxane) Supelco capillary column. GC conditions were: 7C/min temperature ramp from 180C to 210C, followed by a 30C/min ramp to 265C and a 3min hold at 265C. Injector temperature was set to 265C, the flame ionization detector was heated to 250C, Helium flow through the column was 1 mL per min, the split ratio was 100:1 and the septum purge was 4 mL per min. A standard GLC mix of saturated and unsaturated fatty acid methyl esters (Nu-Check Prep) was used to identify retention times
Fatty acid percentages of oil in seeds were determined by GC after converting the fatty acids to methyl esters. 50 seeds per accession were ground in 5 mL of 0.25 M sodium methoxide solution in 20 mL scintillation vials with a Finemech homogenizer for 1 min or until no whole seeds were present. Vials were sealed with aluminum lined screw caps and placed in a 60oC aluminum heating block for 30 min. After removing the vials from the block, 5 mL each of hexane and saturated sodium chloride were added and the contents thoroughly mixed. After the layers separated, 0.5mL aliquots from the top hexane layers were placed into 2 mL GC target vials, diluted to 2 mL with hexane and sealed with crimp caps. The vials were placed in the GC autosampler and injected onto the capillary column of a Hewlett Packard 5890 Series II gas chromatograph (GC) equipped with a flame-ionization detector and a 7963 autosampler/injector. GC separations were obtained on a SP-2380 30m X 0.25mm i.d. poly (90% biscyanopropyl/10% cyanopropylphenyl siloxane) Supelco capillary column. GC conditions were: 7C/min temperature ramp from 180C to 210C, followed by a 30C/min ramp to 265C and a 3min hold at 265C. Injector temperature was set to 265C, the flame ionization detector was heated to 250C, Helium flow through the column was 1 mL per min, the split ratio was 100:1 and the septum purge was 4 mL per min. A standard GLC mix of saturated and unsaturated fatty acid methyl esters (Nu-Check Prep) was used to identify retention times
Total oil content was determined by Pulsed Nuclear Magnetic Resonance (pNMR) on a Bruker mq-CU 20-series, Firmware v 2.50Rev.00 using factory instrument setting 909_18A, NMR frequency 19.98 MHz at 40C and pNMR instrument settings of: digital bandwidth 20,000kHZ; gain 63dB; 16 scans with 2 sec recycle delay; pulse separation 3.5ms with sampling window of 6.9583 - 7.0583ms. Seed samples weighed to the tenth mg were placed into Pyrex No. 9820 vials and heated to 40C for 2 hr before analysis. Response factors for each sample were measured and reported to the tenth mg of oil based on standard curves developed using canola oil.
Total oil content was determined by Pulsed Nuclear Magnetic Resonance (pNMR) on a Bruker mq-CU 20-series, Firmware v 2.50Rev.00 using factory instrument setting 909_18A, NMR frequency 19.98 MHz at 40C and pNMR instrument settings of: digital bandwidth 20,000kHZ; gain 63dB; 16 scans with 2 sec recycle delay; pulse separation 3.5ms with sampling window of 6.9583 - 7.0583ms. Seed samples weighed to the tenth mg were placed into Pyrex No. 9820 vials and heated to 40C for 2 hr before analysis. Response factors for each sample were measured and reported to the tenth mg of oil based on standard curves developed using canola oil.
Documentation from information on accessions file. Original passport documentation and external increases.