VETCH

Collection pictures taken in 2000 from the Kazakstan trip made by Rich Hannan, Stephanie Greene, A. Khusainov, A. Afonin, and N. Dzyubenko.

2003 collection trip photos to Arizona, United States. Phaseolus, Vicia were the main genera collected

Collection pictures from Fred Meuhlbauer and Walt Kaiser 2004 trip to Georgia.

For a Powerpoint view of 24 pictures of the Georgia collection click here. It is 7793 KB in size.

Collection pictures taken in 2006 from the Tajikistan trip by Barbara Hellier, Kenneth Street, Zebuniso Muminshoeva, Farkhod Kosimov, Shakhlo Safarzoda, John Sheppard, Natalya Rukhkyan and Sergey Shuvalov.

Tajikistan collection trip map.

Pictures taken in 2005 from field growing plants at Pullman, Washington

Images of seedling were taken in 2012 from seed obtained from the National Germplasm System (NPGS) and also of many other native forbs of the Inland Northwest. You can find the Forb Seedling Identication Guide here .

Images taken at Whitlow farm at Pullman Washington

Accessions were planted on April 30, 2007 at Whitlow farm near Pullman, Washington. Elevation 2500 feet, Latitude 46 degrees, 43 minutes, 55 seconds, Longitude 117 degrees, 9 minutes, 25 seconds.

Aerial photo of Whitlow farm in Pullman, Wa of the 2007 nursery.

Pullman weather data from September 1, 2006 to August 31, 2007 as an Excel file .

Accessions were planted on April, 2008 at Whitlow farm near Pullman, Washington. Elevation 2500 feet, Latitude 46 degrees, 43 minutes, 55 seconds, Longitude 117 degrees, 9 minutes, 25 seconds.

For a Excel file of the 2008 data

Accessions were planted on April, 2010 at Whitlow farm near Pullman, Washington. Elevation 2500 feet, Latitude 46 degrees, 43 minutes, 55 seconds, Longitude 117 degrees, 9 minutes, 25 seconds.

Accessions were planted on April 25, 2017 (day 115 of the year) at Whitlow farm near Pullman, Washington. Elevation 2500 feet, Latitude 46 degrees, 43 minutes, 55 seconds, Longitude 117 degrees, 9 minutes, 25 seconds.

Vicia sativa seeds. HCN evolved from at least one seed from the accessions after addition of enzyme needed for cyanide production. YES, equals a positive reaction when (B-glucosidase and B-glucuronidase) was added.

Vicia sativa seeds. HCN evolved from at least one seed from the accessions after addition of enzyme needed for cyanide production. YES, equals a positive reaction when (B-glucosidase and B-glucuronidase) was added.

Data came from the Southern Regional Plant Introduction Station (S9)

Vicia sativa seeds, 3 reps, 12 per rep, subjected to the Fiegl_anger test(Kakes 1991). Intensity scores: 0 = acyangenic, 1 least intense, 9 = most intense.

Vicia sativa seeds, 3 reps, 12 per rep, subjected to the Fiegl_anger test(Kakes 1991). Intensity scores: 0 = acyangenic, 1 least intense, 9 = most intense.

Vicia sativa plants, percent cyangenic plants on 12 plants grown 4-6 weeks in the greenhouse. The picric acid test(Hogg and Ahlgren, 1942) w as used to detect cyangensis.

Vicia sativa plants, percent cyangenic plants on 12 plants grown 4-6 weeks in the greenhouse. The picric acid test(Hogg and Ahlgren, 1942) w as used to detect cyangensis.

Vicia sativa seeds, 3 reps, 12 per rep, soaked in distilled water for 24 hours then subjected to the Feigl-Anger test(Kakes 1991)

Vicia sativa seeds, 3 reps, 12 per rep, soaked in distilled water for 24 hours then subjected to the Feigl-Anger test(Kakes 1991)

Data came from the Southern Regional Plant Introduction Station (S9)

Data came from the Southern Regional Plant Introduction Station (S9)

Accessions were planted on April, 2008 at Whitlow farm near Pullman, Washington. Elevation 2500 feet, Latitude 46 degrees, 43 minutes, 55 seconds, Longitude 117 degrees, 9 minutes, 25 seconds.

For a Excel file of the 2008 data

Accessions were planted on April, 2012 at Whitlow farm near Pullman, Washington. Elevation 2500 feet, Latitude 46 degrees, 43 minutes, 55 seconds, Longitude 117 degrees, 9 minutes, 25 seconds.

Data came from the Southern Regional Plant Introduction Station (S9)

The purpose of this research was to determine the presence of ploidy level differentiation of hairy vetch (Vicia villosa) germplasm. Flow cytometry and root tip chromosome squashing methods were employed to assess 45 accessions labeled V. villosa available through the USDA germplasm collection. Flow cytometry determined that 43 of the accessions were 2C, one accession was 4C, and one accession was 6C. Analysis of accessions by root tip chromosome counts indicated that all accessions were diploid. The 2C accession contains 14 chromosomes and their chromosomes were approximately one-half and one-third in size as compared to the chromosomes of the 4C and 6C accessions, respectively. The 4C accession was observed to have 16 chromosomes and the 6C accession was observed to have 14 chromosomes. The large-scale differences in DNA amounts were due to chromosomal size variability as opposed to ploidy differences. This revealed the incidence of species misidentification of these two V. villosa accessions to be Vicia pannonica. All the V. villosa accessions were observed to be diploid and have similar DNA amounts. Flow cytometry proved to be useful in the efficient assessment of these accessions.

Root length in millimeters on Vicia sativa seedlings done by J.A. Mosjidis, Auburn University, Auburn, Alabama, 1998. The method of Mosjidis and Zhang (1995) was used to study differences in root growth among accessions. Seed of the accessions were germinated in a 178 mm high x 166 mm wide polyethylene growth pouch (Seed-Pack, Vaughan's Seed Company), which contain an absorbent paper wick with 21 ml of water. The top of the wick was folded into a V-shaped trough for holding seeds. Perforations in the bottom of the trough facilitate root penetration (McMichael et al., 1985). Four seeds of each accession were placed in a growth pouch. These pouches were held upright in plastic containers and kept in the dark in an incubator at 20 oC. The experimental design was a randomized complete block with eight replications (runs). Main root length of each seedling was measured 12 days after the seeds were put in the incubators. Data was recorded as root length in each accession.

Data came from the Southern Regional Plant Introduction Station (S9)

100 seed weight at the Western Regional Plant Introduction Station, Pullman Washington, USA

One hundred seventy-six seeds per accession were planted in individual Jiffy pellets (Hummert Seed Company, Earth City, Mo) in August 2000 and 2001. They germinated in flats in the greenhouse. Four weeks after planting, the V. villosa plant material was transplanted to field plots in Urbana, IL. The V. pannonica plant material was transplanted to field plots 6 wk after planting in the greenhouse. The field design was a randomized complete block with two blocks. In each block, there were 44 plots measuring 2 by 1 m. Each plot represented an individual accession, and there were 50 plants transplanted to each plot. The individual plants were planted in a five columns by 10 rows lattice with 200 mm between each plant. This was conducted in 2 yr. Initial seed weight for 100 seeds per accession was measured. Percentage germination was calculated for each accession on the basis of the number of plants germinated/total seeds planted in the jiffy pellets. Stem length, stem width, leaf length, and leaf width were measured with a caliper to record fall growth in late October. These measurements were taken on five random plants per accession plot. The leaf measurements were taken near the base of the plant on the third leaflet from the tendril. Stem measurements were taken on the main stems. Ten leaf and stem measurements were taken from each plant. In late April 2001 and April 2002, several additional measurements were recorded in the field for each accession. Winter survival percentages for each accession were determined by number of plants alive/number of plants transplanted. Five plants per plot (if not winter-killed) were randomly selected to measure stem length, stem width, leaf length, and leaf width, with the same methods as the fall measurements. Additionally, a random (30 by 60 cm) section of each plot was cut with hand clippers and weighed for biomass accumulation. Standing residues were cut at ground level with electric shears and the plant material was gathered by hand. Residue samples were dried for 3 d at 65?C, weighed, and ground to pass through a 1-mm mesh. Residue samples were analyzed for total C and N with an automated Dumas instrument (Leco CHN-2000. Leco Corp., St. Joseph, MI). These data were included in the statistical analysis as a ratio of C to N (C:N ratio).

For an Excel file of the 2000 and 2001 data.