| COMMENT | REG_COMPLIANT | REG_COMPLIANT | UNTESTED - Pending compliance testing with 2021 Department of Agriculture Agricultural Marketing Service Hemp Final Rule (7 CFR Part 990 [Doc. No. AMS-SC-19-0042; SC19-990-2 FR]) | | Hemp Final Rule (7 CFR Part 990 [Doc. No. AMS-SC-19-0042; SC19-990-2 FR])">HEMP_REG_COMPLIANCE | | Not Available |
| DISEASE | SEPTORIA | Severity of septoria infestation. TBD 20231006. Update with protocol when available [zjs] | 5 | 2 | 2024-HA24-PGRU-SEQUENCING-Z1 | G 33733 23UO02 SD | Not Available |
| DISEASE | POWDERY | Severity of powdery mildew infestation. TBD 2024112. Update with protocol when available [zjs] | 13.33 | 3 | 2024-HA24-PGRU-SEQUENCING-Z1 | G 33733 23UO02 SD | Not Available |
| FLWR-FRUIT | FEMALE_RATIO | Female ratio. Ratio of observed population with only pistillate flowers; ratio pistillate only individuals observed (0-100). | 0.64 | 56 | ROUTINE.REGENERATION.HEMP | G 33733 23UO02 SD | Not Available |
| FLWR-FRUIT | FEMALE_RATIO | Female ratio. Ratio of observed population with only pistillate flowers; ratio pistillate only individuals observed (0-100). | 0.75 | 9 | 2024-HA24-PGRU-SEQUENCING-Z1 | G 33733 23UO02 SD | Not Available |
| FLWR-FRUIT | MALE_RATIO | Male ratio. Ratio of observed population with only staminate flowers; Ratio staminate only individuals observed | 0.25 | 9 | 2024-HA24-PGRU-SEQUENCING-Z1 | G 33733 23UO02 SD | Not Available |
| FLWR-FRUIT | MALE_RATIO | Male ratio. Ratio of observed population with only staminate flowers; Ratio staminate only individuals observed | 0.36 | 56 | ROUTINE.REGENERATION.HEMP | G 33733 23UO02 SD | Not Available |
| FLWR-FRUIT | MONO_RATIO | Monoecious ratio. Ratio of observed population both pistillate and staminate flowers. | 0 | 9 | 2024-HA24-PGRU-SEQUENCING-Z1 | G 33733 23UO02 SD | Not Available |
| FLWR-FRUIT | MONO_RATIO | Monoecious ratio. Ratio of observed population both pistillate and staminate flowers. | 0 | 56 | ROUTINE.REGENERATION.HEMP | G 33733 23UO02 SD | Not Available |
| FLWR-FRUIT | F_DAYLENGTH | Female (daylength). Daylength at terminal female flowering. Calculated using site data for latitude, in degrees (i.e. between -90.0 and 90.0) with the daylength() function in the R package geosphere (https://rdrr.io/cran/geosphere/man/daylength.html). [hours]. | 13.94 | 6 | 2024-HA24-PGRU-SEQUENCING-Z1 | G 33733 23UO02 SD | Not Available |
| FLWR-FRUIT | M_DAYLENGTH | Male (daylength). Daylength at first male flower anthesis. Calculated using site data for latitude, in degrees (i.e. between -90.0 and 90.0) with the daylength() function in the R package geosphere (https://rdrr.io/cran/geosphere/man/daylength.html). | 14.45 | 3 | 2024-HA24-PGRU-SEQUENCING-Z1 | G 33733 23UO02 SD | Not Available |
| GENSTOCK | SEED.NO | Number of seeds in original seed lot (count) | 121 | | MARVIN_SEED_ANALYSIS_PGRU_V001 | G 33733 23UO02 SD | Not Available |
| GENSTOCK | SEED.NO | Number of seeds in original seed lot (count) | 67 | | MARVIN_SEED_ANALYSIS_PGRU_V001 | G 33733 23UO01 SD | Not Available |
| GENSTOCK | SEED.WEIGHT | Seed Weight (g) | 0.89 | | MARVIN_SEED_ANALYSIS_PGRU_V001 | G 33733 23UO01 SD | Not Available |
| GENSTOCK | SEED.WEIGHT | Seed Weight (g) | 1.39 | | MARVIN_SEED_ANALYSIS_PGRU_V001 | G 33733 23UO02 SD | Not Available |
| GENSTOCK | TSW | Thousand seed weight (g) | 11.49 | | MARVIN_SEED_ANALYSIS_PGRU_V001 | G 33733 23UO02 SD | Not Available |
| GENSTOCK | TSW | Thousand seed weight (g) | 13.28 | | MARVIN_SEED_ANALYSIS_PGRU_V001 | G 33733 23UO01 SD | Not Available |
| GENSTOCK | NON.SEED | Percent of non-seed in sample (%) | 1.5 | | MARVIN_SEED_ANALYSIS_PGRU_V001 | G 33733 23UO01 SD | Not Available |
| GENSTOCK | SEED.AREA | SEED.AREA | 7.8 | | MARVIN_SEED_ANALYSIS_PGRU_V001 | G 33733 23UO02 SD | Not Available |
| GENSTOCK | SEED.AREA | SEED.AREA | 8.6 | | MARVIN_SEED_ANALYSIS_PGRU_V001 | G 33733 23UO01 SD | Not Available |
| GENSTOCK | SEED.WIDTH | Seed Width (mm) | 2.8 | | MARVIN_SEED_ANALYSIS_PGRU_V001 | G 33733 23UO02 SD | Not Available |
| GENSTOCK | SEED.WIDTH | Seed Width (mm) | 2.9 | | MARVIN_SEED_ANALYSIS_PGRU_V001 | G 33733 23UO01 SD | Not Available |
| GENSTOCK | SEED.LENGTH | Seed length (mm) | 3.8 | | MARVIN_SEED_ANALYSIS_PGRU_V001 | G 33733 23UO02 SD | Not Available |
| GENSTOCK | SEED.LENGTH | Seed length (mm) | 3.9 | | MARVIN_SEED_ANALYSIS_PGRU_V001 | G 33733 23UO01 SD | Not Available |
| GENSTOCK | SEED.LEN.WIDTH.RATIO | Seed length to width ratio | 1.4 | | MARVIN_SEED_ANALYSIS_PGRU_V001 | G 33733 23UO01 SD | Not Available |
| GENSTOCK | SEED.LEN.WIDTH.RATIO | Seed length to width ratio | 1.4 | | MARVIN_SEED_ANALYSIS_PGRU_V001 | G 33733 23UO02 SD | Not Available |
| GENSTOCK | SEED.BRIGHTNESS | Seed brightness | 43.3 | | MARVIN_SEED_ANALYSIS_PGRU_V001 | G 33733 23UO01 SD | Not Available |
| GENSTOCK | SEED.BRIGHTNESS | Seed brightness | 43.7 | | MARVIN_SEED_ANALYSIS_PGRU_V001 | G 33733 23UO02 SD | Not Available |
| MOLECULAR | CCP1.ratio | CCP1.ratio | I:1/II:2/III:13 | 56 | ROUTINE.REGENERATION.HEMP | G 33733 23UO02 SD | Not Available |
| MOLECULAR | CSP1.ratio | CSP1.ratio | 17XX/12XY/21DNA | 56 | ROUTINE.REGENERATION.HEMP | G 33733 23UO02 SD | Not Available |
| MORPHOLOGY | MCD | Maximum canopy diameter. [decimal; cm] Maximum canopy diameter (mcd) as width of plant at widest set of branches, see Carlson et al. (2021). Measured from widest tip to tip without stretching branches. Include flowering tissue in measurement. | 126.49 | 4 | 2024-HA24-PGRU-SEQUENCING-Z1 | G 33733 23UO02 SD | Not Available |
| MORPHOLOGY | MCDH | Height evaluated at maximum canopy diameter. [decimal; cm] Maximum canopy diameter height, Evaluated at maximum canopy diameter (MCD) to ground, see Carlson et al. (2021). | 97.84 | 4 | 2024-HA24-PGRU-SEQUENCING-Z1 | G 33733 23UO02 SD | Not Available |
| MORPHOLOGY | TRKL | Trunk length. [decimal; cm] Trunk length (trkl) is evaluated as distance from ground to first branch. See Carlson et al. (2021). | 8.125 | 5 | 2024-HA24-PGRU-SEQUENCING-Z1 | G 33733 23UO02 SD | Not Available |
| MORPHOLOGY | KITE_PERIMETER | Kite Perimeter. [decimal; cm] See Carlson et al. (2021). | 367.652 | 5 | 2024-HA24-PGRU-SEQUENCING-Z1 | G 33733 23UO02 SD | Not Available |
| MORPHOLOGY | KITE_AREA | Kite Area. [decimal; cm2] See Carlson et al. (2021). | 7335.86 | 5 | 2024-HA24-PGRU-SEQUENCING-Z1 | G 33733 23UO02 SD | Not Available |
| MORPHOLOGY | DIA | Diameter of the stem. dia [decimal; mm]
Diameter of the stem at soil level using forestry measuring tape or fabric measuring tape, modified from Carlson et al. (2021). | 38.575 | 5 | 2024-HA24-PGRU-SEQUENCING-Z1 | G 33733 23UO02 SD | Not Available |
| MORPHOLOGY | KITE_CIRCULARITY | Kite Circularity. A continuous scale of apical dominance can be derived (See Carlson et al. 2021) | 0.67 | 5 | 2024-HA24-PGRU-SEQUENCING-Z1 | G 33733 23UO02 SD | Not Available |
| MORPHOLOGY | HT | see https://www.ars.usda.gov/northeast-area/geneva-ny/plant-genetic-resources-unit-pgru/docs/hemp-descriptors/ | 115.06 | 4 | 2024-HA24-PGRU-SEQUENCING-Z1 | G 33733 23UO02 SD | Not Available |
| MORPHOLOGY | TRK_RATIO | Test phenotype calculated as the ratio of trunk length divided by the height of the plant [TRKL/HT]. This is a proxy for the degree of branching on the lower portion of the plant, which has been identified in conversations as an important agronomic trait for fiber production. PGRU is evaluating this trait, but it is not currently included in the Hemp Phenotyping Handbook. | 0.07708 | 5 | 2024-HA24-PGRU-SEQUENCING-Z1 | G 33733 23UO02 SD | Not Available |
| PHENOLOGY | DAYS_TO_FLOWER_FEMALE | Days To Flower Female. [integer]
Pre-terminal date when axial flowers with shortening internodes and terminal pistils (clusters of flowers at shoot termini) were observed Carlson et al. (2021) as days from sowing to first observed open female and male inflorescence. See also Faux et al. (2013); Shams et al. (2020) | 100 | 6 | 2024-HA24-PGRU-SEQUENCING-Z1 | G 33733 23UO02 SD | Not Available |
| PHENOLOGY | DAYS_TO_FLOWER_MALE | Days To Flower Male . [integer]
Pre-terminal date when axial flowers with shortening internodes and terminal pistils (clusters of flowers at shoot termini) were observed Carlson et al. (2021) as days from sowing to first observed open female and male inflorescence. See also Faux et al. (2013); Shams et al. (2020) | 87.5 | 2 | 2024-HA24-PGRU-SEQUENCING-Z1 | G 33733 23UO02 SD | Not Available |
| PHENOLOGY | DAY_NEUTRALITY | Day Neutrality. [nvarchar]
Describe flowering behavior as critical day length and/or day neutral response. A more precise definition is required to define this phenotype quantitatively. | 0 | 9 | 2024-HA24-PGRU-SEQUENCING-Z1 | G 33733 23UO02 SD | Not Available |