Description:
L-74X traces to an initial 1998/1990 interspecific F1 cross (Leymus cinereus female x L. triticoides male) between multiple plants of M10 (USDA Acc:636), an L. cinereus population received from Sylvester Smoliak (Agriculture Canada, Lethbridge, AB), and A30 (PI 537359; USDA Acc:641), an L. triticoides population collected by Kay Asay (USDA-ARS, Logan, UT) near Jamieson, OR. In 1992, the F2 progeny of the cross were generated. Eight generations of recombination occurred following the initial hybridization, which facilitated the restoration of fertility in the interspecific hybrid population. Partway through this process, a controlled biparental cross of two individual L-57S plants, L-57S-491 (sh6/sh6) x L-57S-577 (SH6/sh6) was made to test and validate linkage of the Ltc096.158 marker and the sh6 seed retention gene. This created a genetic bottleneck, severely reducing genetic variation. and necessitating a germplasm rather than a cultivar release. In the final generation, 70 non-shattering (sh6/sh6) selections were intermated and harvested for seed, constituting L-74X.