Description:
The original population was developed by crossing C1805-2-9-16 x N08082olJCT. C1805-2-9-16 is a nematode resistant breeding line selected from the cross of ‘Tifguard’ x ‘Florida-07’. N08082olJCT is a ‘Bailey’ derived high oleic breeding line. F1 plants were grown in the summer of 2012, and the F2 population was grown during the summer of 2013. Marker assisted selection was used to identify F2 plants (eg. 13-3532) that were homozygous for nematode resistance (Rma) and high oleic acid content, and these plants were individually harvested. For nematode resistance conferred by Rma, dominant marker S197 (resistant allele), dominant CAPS marker 1169/1170 (susceptibility allele), and a codominant simple sequence repeat (SSR) marker GH565 were used. Cleaved amplified polymorphic sequence marker 1101/1048 targeting the 441_442insA mutation of AhFAD2 was used to genotype for the high O/L trait. In addition, this CAPS marker was converted to a gel-free single nucleotide polymorphism (SNP) assay using a HybProbe design. The AhFAD2B mutant allele was submitted to LightCycler Probe Design software 2.0 (Roche Applied Science, 2004) for primer and probe design. F2:3 progeny rows were grown during the summer of 2014, and visually evaluated for resistance to TSWV and agronomic characteristics before and after digging. Individual plants in the top families were harvested and sent to the Puerto Rico winter nursery for seed increase. Replicated yield testing began with F3:5 material. Molecular markers were used to identify F7 plants that were homogeneous for nematode resistance and high oleic acid content for use in further seed increase. F7:9 material was increased in 2019 in Tifton, GA. Harvested seed were sent to the 2019/2020 winter nursery for additional seed increase.