Narrative
QTL6/CU17NBL is a line derived in the CU17NBL acylsugar benchmark breeding line by transferring the QTL6 introgressions, which increases acylsugar level and increases the density of Type IV trichomes which produce and exude acylsugars.
The creation of the major Cornell acylsugar lines including CU071026 and CU17NBL is reviewed in the publication by Mutschler (2021).
CU17NBL has 5 S. pennellii introgressions which combined are ca. 11% of the CU17NBL genome. The locations and sizes of these locations using the 2.5 genome build are:
• Chromosome 2: the Chr2 introgression, from 53,792,960 - 55,238,304 bp: Therefore, it is 1,445,344 bp in length.
• Chromosome 3: the Chr3 introgression, from 2,143,744 - 64,451,474 bp: Therefore, it is 62,307,730 bp length.
• Chromosome 7: the Chr7 introgression, from 65,554,074 – 65,801,666 bp: Therefore, it is 247,592 bp in length.
• Chromosome 8: the Chr8 introgression, from 60,843,834 – 63,384,503 bp: Therefore, it is 2,540,669 bp in length
• Chromosome 10: the Chr10U introgression, from 0 - 1,916,909 bp: Therefore, it is 1,916,909 bp in length.
The additional introgression of QTL6/CU17NBL is:
• Chromosome 6: the Chr6 introgression, from 44,996,740 to 49,632,974 bp. Therefore, it is ca. 4,636,234bp in length.
A variant of the QTL6/CU17NBL line, named QTL6/Sw-5/CUNBL, was also created that is the same as QTL6/CU17NBL except that also has an introgression containing the Sw-5 gene for resistance to TSWV. Seed of QTL6/Sw-5/CU17NBL has also been deposited.
The source of the QTL6 and the Sw-5 containing introgressions to create QTL6/CU17NBL and QTL6/Sw-5/CU17NBL lines was the analogous line in CU071026, QTL6/Sw-5/ CU071026.
The QTL6/CU17NBL and QTL6/Sw-5/CU17NBL lines are very similar in acylsugar level and acylsugar types to that of their analogous lines in CU071026 background: QTL6/ CU071026 and QTL6/Sw-5/ CU071026 lines. The impacts of increased acylsugar level of QTL6/ CU071026 and QTL6/Sw-5/ CU071026 lines on Bemisia whitefly oviposition is in Leckie et al (2012). A publication regarding creation and complete characterizations of QTL6/CU17NBL and QTL6/Sw-5/CU17NBL, and other variant lines in CU17NBL is in preparation.