| CHEMICAL | cyanidin.3.O.rutinoside | Flavinol analysis of hemp germplasm. Collected from floral tissue 35 days post terminal flowering. Seed phenotyping handbook for flavinoid protocol: https://www.ars.usda.gov/northeast-area/geneva-ny/plant-genetic-resources-unit-pgru/docs/hemp-descriptors/ | 0 | | 2022-PGRU-RN010-FLAVINOID | | Not Available |
| CHEMICAL | orientin | Flavinol analysis of hemp germplasm. Collected from floral tissue 35 days post terminal flowering. Seed phenotyping handbook for flavinoid protocol: https://www.ars.usda.gov/northeast-area/geneva-ny/plant-genetic-resources-unit-pgru/docs/hemp-descriptors/ | 6023.38 | | 2022-PGRU-RN010-FLAVINOID | | Not Available |
| CHEMICAL | luteolin.7.O.glucuronide | Flavinol analysis of hemp germplasm. Collected from floral tissue 35 days post terminal flowering. Seed phenotyping handbook for flavinoid protocol: https://www.ars.usda.gov/northeast-area/geneva-ny/plant-genetic-resources-unit-pgru/docs/hemp-descriptors/ | 6385.66 | | 2022-PGRU-RN010-FLAVINOID | | Not Available |
| CHEMICAL | apigenin.7.O.glucuronide | Flavinol analysis of hemp germplasm. Collected from floral tissue 35 days post terminal flowering. Seed phenotyping handbook for flavinoid protocol: https://www.ars.usda.gov/northeast-area/geneva-ny/plant-genetic-resources-unit-pgru/docs/hemp-descriptors/ | 0 | | 2022-PGRU-RN010-FLAVINOID | | Not Available |
| COMMENT | REG_COMPLIANT | REG_COMPLIANT | UNTESTED - Pending compliance testing with 2021 Department of Agriculture Agricultural Marketing Service Hemp Final Rule (7 CFR Part 990 [Doc. No. AMS-SC-19-0042; SC19-990-2 FR]) | | Hemp Final Rule (7 CFR Part 990 [Doc. No. AMS-SC-19-0042; SC19-990-2 FR])">HEMP_REG_COMPLIANCE | | Not Available |
| FLWR-FRUIT | FEMALE_RATIO | Female ratio. Ratio of observed population with only pistillate flowers; ratio pistillate only individuals observed (0-100). | 0 | 1 | 2022-SAC-USDA-PGRU-RN010-MORPHOLOGY | G 33328 21UO SD | Not Available |
| FLWR-FRUIT | FEMALE_RATIO | Female ratio. Ratio of observed population with only pistillate flowers; ratio pistillate only individuals observed (0-100). | 0 | 9 | 2024-HA24-PGRU-SEQUENCING-Z1 | G 33328 21UO SD | Not Available |
| FLWR-FRUIT | MALE_RATIO | Male ratio. Ratio of observed population with only staminate flowers; Ratio staminate only individuals observed | 1 | 1 | 2022-SAC-USDA-PGRU-RN010-MORPHOLOGY | G 33328 21UO SD | Not Available |
| FLWR-FRUIT | MALE_RATIO | Male ratio. Ratio of observed population with only staminate flowers; Ratio staminate only individuals observed | 1 | 9 | 2024-HA24-PGRU-SEQUENCING-Z1 | G 33328 21UO SD | Not Available |
| FLWR-FRUIT | MONO_RATIO | Monoecious ratio. Ratio of observed population both pistillate and staminate flowers. | 0 | 1 | 2022-SAC-USDA-PGRU-RN010-MORPHOLOGY | G 33328 21UO SD | Not Available |
| FLWR-FRUIT | MONO_RATIO | Monoecious ratio. Ratio of observed population both pistillate and staminate flowers. | 0 | 9 | 2024-HA24-PGRU-SEQUENCING-Z1 | G 33328 21UO SD | Not Available |
| FLWR-FRUIT | M_FLOW_DATE | Male flowering date. Date of staminate flower initiation | 2022-07-20 | | 2022-SAC-USDA-PGRU-RN010-MORPHOLOGY | G 33328 21UO SD | Not Available |
| FLWR-FRUIT | M_DAYLENGTH | Male (daylength). Daylength at first male flower anthesis. Calculated using site data for latitude, in degrees (i.e. between -90.0 and 90.0) with the daylength() function in the R package geosphere (https://rdrr.io/cran/geosphere/man/daylength.html). | 14.93 | | 2022-SAC-USDA-PGRU-RN010-MORPHOLOGY | G 33328 21UO SD | Not Available |
| FLWR-FRUIT | M_DAYLENGTH | Male (daylength). Daylength at first male flower anthesis. Calculated using site data for latitude, in degrees (i.e. between -90.0 and 90.0) with the daylength() function in the R package geosphere (https://rdrr.io/cran/geosphere/man/daylength.html). | 15.04 | 2 | 2024-HA24-PGRU-SEQUENCING-Z1 | G 33328 21UO SD | Not Available |
| GENSTOCK | SEED.WEIGHT | Seed Weight (g) | 1.4 | | MARVIN_SEED_ANALYSIS_PGRU_V001 | G 33328 21UO SD | Not Available |
| GENSTOCK | TSW | Thousand seed weight (g) | 14 | | MARVIN_SEED_ANALYSIS_PGRU_V001 | G 33328 21UO SD | Not Available |
| GENSTOCK | SEED.AREA | SEED.AREA | 8.7 | | MARVIN_SEED_ANALYSIS_PGRU_V001 | G 33328 21UO SD | Not Available |
| GENSTOCK | SEED.WIDTH | Seed Width (mm) | 3 | | MARVIN_SEED_ANALYSIS_PGRU_V001 | G 33328 21UO SD | Not Available |
| GENSTOCK | SEED.LENGTH | Seed length (mm) | 3.9 | | MARVIN_SEED_ANALYSIS_PGRU_V001 | G 33328 21UO SD | Not Available |
| GENSTOCK | SEED.LEN.WIDTH.RATIO | Seed length to width ratio | 1.3 | | MARVIN_SEED_ANALYSIS_PGRU_V001 | G 33328 21UO SD | Not Available |
| GENSTOCK | SEED.BRIGHTNESS | Seed brightness | 41.2 | | MARVIN_SEED_ANALYSIS_PGRU_V001 | G 33328 21UO SD | Not Available |
| MORPHOLOGY | MCD | Maximum canopy diameter. [decimal; cm] Maximum canopy diameter (mcd) as width of plant at widest set of branches, see Carlson et al. (2021). Measured from widest tip to tip without stretching branches. Include flowering tissue in measurement. | 143.26 | 1 | 2022-SAC-USDA-PGRU-RN010-MORPHOLOGY | G 33328 21UO SD | Not Available |
| MORPHOLOGY | MCD | Maximum canopy diameter. [decimal; cm] Maximum canopy diameter (mcd) as width of plant at widest set of branches, see Carlson et al. (2021). Measured from widest tip to tip without stretching branches. Include flowering tissue in measurement. | 176.78 | 1 | 2024-HA24-PGRU-SEQUENCING-Z1 | G 33328 21UO SD | Not Available |
| MORPHOLOGY | MCDH | Height evaluated at maximum canopy diameter. [decimal; cm] Maximum canopy diameter height, Evaluated at maximum canopy diameter (MCD) to ground, see Carlson et al. (2021). | 36.58 | 1 | 2024-HA24-PGRU-SEQUENCING-Z1 | G 33328 21UO SD | Not Available |
| MORPHOLOGY | MCDH | Height evaluated at maximum canopy diameter. [decimal; cm] Maximum canopy diameter height, Evaluated at maximum canopy diameter (MCD) to ground, see Carlson et al. (2021). | 94.49 | 1 | 2022-SAC-USDA-PGRU-RN010-MORPHOLOGY | G 33328 21UO SD | Not Available |
| MORPHOLOGY | TRKL | Trunk length. [decimal; cm] Trunk length (trkl) is evaluated as distance from ground to first branch. See Carlson et al. (2021). | 11 | 1 | 2022-SAC-USDA-PGRU-RN010-MORPHOLOGY | G 33328 21UO SD | Not Available |
| MORPHOLOGY | TRKL | Trunk length. [decimal; cm] Trunk length (trkl) is evaluated as distance from ground to first branch. See Carlson et al. (2021). | 11.2 | 2 | 2024-HA24-PGRU-SEQUENCING-Z1 | G 33328 21UO SD | Not Available |
| MORPHOLOGY | INL | [decimal; cm] Average internode length (inl) is calculated between all branching pairs along 50 cm of the primary stem. See Carlson et al. (2021). | 7.9 | 1 | 2022-SAC-USDA-PGRU-RN010-MORPHOLOGY | G 33328 21UO SD | Not Available |
| MORPHOLOGY | KITE_PERIMETER | Kite Perimeter. [decimal; cm] See Carlson et al. (2021). | 533.72 | 1 | 2022-SAC-USDA-PGRU-RN010-MORPHOLOGY | G 33328 21UO SD | Not Available |
| MORPHOLOGY | KITE_PERIMETER | Kite Perimeter. [decimal; cm] See Carlson et al. (2021). | 614.104 | 2 | 2024-HA24-PGRU-SEQUENCING-Z1 | G 33328 21UO SD | Not Available |
| MORPHOLOGY | KITE_AREA | Kite Area. [decimal; cm2] See Carlson et al. (2021). | 16068.51 | 1 | 2022-SAC-USDA-PGRU-RN010-MORPHOLOGY | G 33328 21UO SD | Not Available |
| MORPHOLOGY | KITE_AREA | Kite Area. [decimal; cm2] See Carlson et al. (2021). | 20206.41 | 2 | 2024-HA24-PGRU-SEQUENCING-Z1 | G 33328 21UO SD | Not Available |
| MORPHOLOGY | BRANCHES | Number of branches per plant. [int] Number of branches per plant. When grown from seed, branching is initially opposite, transitioning to alternate as the plant matures. Plants propagated from cuttings generally have alternate branching in the whole plant Stack et al. (2021). Modified Carlson et al. (2021). | 27 | 1 | 2022-SAC-USDA-PGRU-RN010-MORPHOLOGY | G 33328 21UO SD | Not Available |
| MORPHOLOGY | DIA | Diameter of the stem. dia [decimal; mm]
Diameter of the stem at soil level using forestry measuring tape or fabric measuring tape, modified from Carlson et al. (2021). | 30.88 | 1 | 2022-SAC-USDA-PGRU-RN010-MORPHOLOGY | G 33328 21UO SD | Not Available |
| MORPHOLOGY | DIA | Diameter of the stem. dia [decimal; mm]
Diameter of the stem at soil level using forestry measuring tape or fabric measuring tape, modified from Carlson et al. (2021). | 51.2 | 2 | 2024-HA24-PGRU-SEQUENCING-Z1 | G 33328 21UO SD | Not Available |
| MORPHOLOGY | KITE_CIRCULARITY | Kite Circularity. A continuous scale of apical dominance can be derived (See Carlson et al. 2021) | 0.67 | 2 | 2024-HA24-PGRU-SEQUENCING-Z1 | G 33328 21UO SD | Not Available |
| MORPHOLOGY | KITE_CIRCULARITY | Kite Circularity. A continuous scale of apical dominance can be derived (See Carlson et al. 2021) | 0.71 | 1 | 2022-SAC-USDA-PGRU-RN010-MORPHOLOGY | G 33328 21UO SD | Not Available |
| MORPHOLOGY | LODGE | Lodging. lodging recorded after windstorm | 30 | 1 | 2022-SAC-USDA-PGRU-RN010-MORPHOLOGY | G 33328 21UO SD | Not Available |
| MORPHOLOGY | HT | see https://www.ars.usda.gov/northeast-area/geneva-ny/plant-genetic-resources-unit-pgru/docs/hemp-descriptors/ | 224.33 | 1 | 2022-SAC-USDA-PGRU-RN010-MORPHOLOGY | G 33328 21UO SD | Not Available |
| MORPHOLOGY | HT | see https://www.ars.usda.gov/northeast-area/geneva-ny/plant-genetic-resources-unit-pgru/docs/hemp-descriptors/ | 228.6 | 1 | 2024-HA24-PGRU-SEQUENCING-Z1 | G 33328 21UO SD | Not Available |
| MORPHOLOGY | N_OPP | Number of opposite nodes . see https://www.ars.usda.gov/northeast-area/geneva-ny/plant-genetic-resources-unit-pgru/docs/hemp-descriptors/ | 7 | 1 | 2022-SAC-USDA-PGRU-RN010-MORPHOLOGY | G 33328 21UO SD | Not Available |
| MORPHOLOGY | N_ALT | Number of alternate nodes. | 13 | 1 | 2022-SAC-USDA-PGRU-RN010-MORPHOLOGY | G 33328 21UO SD | Not Available |
| MORPHOLOGY | TRK_RATIO | Test phenotype calculated as the ratio of trunk length divided by the height of the plant [TRKL/HT]. This is a proxy for the degree of branching on the lower portion of the plant, which has been identified in conversations as an important agronomic trait for fiber production. PGRU is evaluating this trait, but it is not currently included in the Hemp Phenotyping Handbook. | 0.04899 | 2 | 2024-HA24-PGRU-SEQUENCING-Z1 | G 33328 21UO SD | Not Available |
| PHENOLOGY | DAYS_TO_FLOWER_MALE | Days To Flower Male . [integer]
Pre-terminal date when axial flowers with shortening internodes and terminal pistils (clusters of flowers at shoot termini) were observed Carlson et al. (2021) as days from sowing to first observed open female and male inflorescence. See also Faux et al. (2013); Shams et al. (2020) | 69 | 1 | 2024-HA24-PGRU-SEQUENCING-Z1 | G 33328 21UO SD | Not Available |
| PHENOLOGY | DAYS_TO_FLOWER_MALE | Days To Flower Male . [integer]
Pre-terminal date when axial flowers with shortening internodes and terminal pistils (clusters of flowers at shoot termini) were observed Carlson et al. (2021) as days from sowing to first observed open female and male inflorescence. See also Faux et al. (2013); Shams et al. (2020) | 71 | 1 | 2022-SAC-USDA-PGRU-RN010-MORPHOLOGY | G 33328 21UO SD | Not Available |
| PHENOLOGY | DAY_NEUTRALITY | Day Neutrality. [nvarchar]
Describe flowering behavior as critical day length and/or day neutral response. A more precise definition is required to define this phenotype quantitatively. | 0 | 1 | 2022-SAC-USDA-PGRU-RN010-MORPHOLOGY | G 33328 21UO SD | Not Available |
| PHENOLOGY | DAY_NEUTRALITY | Day Neutrality. [nvarchar]
Describe flowering behavior as critical day length and/or day neutral response. A more precise definition is required to define this phenotype quantitatively. | 0 | 9 | 2024-HA24-PGRU-SEQUENCING-Z1 | G 33328 21UO SD | Not Available |