Description:
Alborea 301 is derived from sexual hybrids of alfalfa X Medicago arborea. Both species are autotetraploids, 2n=4x=32 chromosomes. Only selected alfalfa genotypes produced hybrids when pollinated with pollen from M. arborea. Yellow flower color from M. arborea was used to identify hybrids, that were later confirmed with DNA analysis. The alfalfa parents were MBms, a male sterile plant from Magnum III X Blaser XL, and M8, a complex 3-way hybrid involving three diverse M. sativa genotypes, and three different tetraploid M. falcata genotypes (WISFAL PI 560333). The M. arborea parents were from PI 199254, 504540, 249937, and 330677. Full details are available on line in Medicago Genetic Reports, Vol. 5 onward, on line Journal: Plants MDPI 2:343-353, and Agricultural Science 28:40-46 (Journal of the Australian Inst. Ag. Sci. and Tech.-not on line). Twenty hybrids were produced since 2006, each retaining 8-15 percent M. arborea germplasm. Hybrids were intercrossed and advanced up to three generations in Alborea 301. M. arborea traits segregating in advanced generations indicate that most of the M. arborea genome is represented in Alborea 301.