Description:
Selected from a TILLING population (Uauy et al., 2009) of 'Kronos' (PI 576168). A detailed description of the identification, selection and generation of the mutant lines is described in Hazard et al. (2012). Mutant lines were backcrossed twice to Kronos to reduce background mutations. SBEIIa and SBEIIb mutations were combined using natural recombination and plants homozygous for the two mutations both in the A (SBEIIa/b-A) and in the B (SBEIIa/b-B) genomes were selected (Hazard et al. 2012). We intercrossed SBEIIa/b-A and SBEIIa/b-B, self-pollinated the F1 hybrid and generated a new segregating population. From this segregating population we selected sib lines homozygous for SBEIIa and SBEIIb mutations only in the A genome (SBEIIa/b-A, PI 670159), only in the B genome (SBEIIa/b-B, PI 670161), in both the A and B genomes (SBEIIa/b-AB, PI 670160) and sib lines with no SBEII mutations to be used as controls.